FastQCFastQC Report
Thu 24 Aug 2023
SRR10811668_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10811668_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135045025
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length10-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGTGGCGGAATTGGTAGACGCGC32882442.434924203983079No Hit
NNNNNNNNNN28556412.11458437658107No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGA27070702.0045684763285427No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGC21433611.587145472408184No Hit
GCGAAGGTGGCGGAATTGGTAGACGCG19400001.4365579183683368No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTG16335201.2096113870170337No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGG14175901.049716566752459No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAC13824091.0236652553472443No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGAT12388550.9173644123506216No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAC10766960.7972866827193374No Hit
GGTGAGGTGTCCGAGTGGCTGAAGGAGC10028890.7426330588631458No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTG9679090.7167305867061745No Hit
TCCTCTGTAGTTCAGTCGGTAGAACG9034950.6690324208537115No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTT8110840.600602650856631No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGA8106480.6002797955718843No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGT7826320.5795341220455918No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATT6756000.5002775926029115No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGC6336970.46924868205992776No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAC6321180.46807944239337956No Hit
TGGCTATAGCTCAGTTGGTAGAGCCCTGGA6110520.4524802005849531No Hit
CCGACTTAGCTCAGTAGGTAGAGCAACTGA5706400.4225553662565503No Hit
GTGAGGTGTCCGAGTGGCTGAAGGAGC5509310.4079609744972093No Hit
GTGGCTATAGCTCAGTTGGTAGAGCCCTGGAT5241690.38814388016144985No Hit
AGGCTTGTAGCTC5162310.3822658405964974No Hit
CCTCTGTAGTTCAGTCGGTAGAACG5030680.372518721070991No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGT4570860.338469336430572No Hit
CCTCTGTAGTTCAGTCGGTAGAACGG4440550.32881996208301634No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGA4430460.3280728038667104No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCT4429910.32803207670923085No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTC4373500.32385495133937736TruSeq Adapter, Index 2 (100% over 50bp)
GGCGCGTTAACAAAGCGGTTATGTAGCGGATT4359810.32284121536502364No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCC4320800.3199525491590675No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCT4210970.3118197060572946No Hit
TGTGGGCACTCGAAGATACGGATTC3929940.2910096095728073No Hit
TGAGGTGTCCGAGTGGCTGAAGGAGC3771890.2793061055007395No Hit
TGTGGGCACTCGAAGATACGGATTCT3727480.27601757265771176No Hit
TGTGGGCACTCGAAGATACGG3544710.26248356798038286No Hit
TGTGGGCACTCGAAGATACGGA3363060.24903249860555765No Hit
TGTGGGCACTCGAAGATACGGATTCTT3276410.2426161200681032No Hit
TGTGGGCACTCGAAGATA3263090.2416297823633266No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACG3253540.24092261081072774No Hit
TGGCTATAGCTCAGTTGGTAGAGCCCTGGAT3026650.22412154760976943No Hit
GAAGGTGGCGGAATTGGTAGACGCG2924400.21654999878744147No Hit
TGTGGGCACTCGAAGATACGGAT2861550.21189599542819146No Hit
CCTCTGTAGTTCAGTCGGTAGAACGGCGGA2747620.20345954987975307No Hit
TGTGGGCACTCGAAGATACG2705640.20035095702340758No Hit
CGGCGGTAACAGGGGTTCGAATCCCCTAGGGGAC2651360.19633155682706566No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGC2614290.19358654641294634No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGC2495230.18477022755928996No Hit
TGTGGGCACTCGAAGATACGGATT2494500.18471617151390804No Hit
GCGGGCGTAGTTCAATGGTAGAACGAGAGC2401030.17779477622370762No Hit
CGAAGGTGGCGGAATTGGTAGACGCG2334140.17284161338042628No Hit
CCTTAAAGAAGCGTACTTTGTAGTGCTCACACA2319030.17172272729039814No Hit
TGTGGGCACTCGAAGATACGGATTCTTA2259080.16728346712513104No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCC2166260.16041020393013367No Hit
GCGAAGGTGGCGGAATTGGTAGACGC2102570.15569399909400589No Hit
GTGGCTATAGCTCAGTTGGTAGAGCCCTGGA2095670.15518305839108104No Hit
AAGGTGGCGGAATTGGTAGACGCG2050650.15184935542793968No Hit
AGGGGCGTAGTTCAATTGGTAGAGCACCGGT1986610.14710723330977948No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGAC1888190.1398192936022634No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACT1841170.13633749188465108No Hit
CCTCTGTAGTTCAGTCGGTAGAACGGC1820510.13480763175096602No Hit
TGTGGGCACTCGAAGATACGGATTCTTAA1761830.13046241429478797No Hit
CGGACGTGGGGGTTCAAGTCCCCCCCCTCGCAC1710310.12664739037961598No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTA1673380.12391274687830965No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTC1634150.12100778980936172No Hit
GCGAAGGTGGCGGAATTGGTAGACG1594560.11807617496460902No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGT1545610.11445145794893222No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACC1481550.10970785484322729No Hit
CGGACGTGGGGGTTCAAGTCCCCCCC1439520.10659555951802No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTA1439160.10656890174221524No Hit
TCCTCTGTAGTTCAG1437000.10640895508738658No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCC1422390.10532709368597622No Hit
CCTTAAAGAAGCGTACTTTGCAGTGCTCACACA1417840.10499016901955478No Hit
ATCTGAGGGTCGGACGTTCGAATCGTTCCGGGTGC1402060.10382166984677889No Hit
GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGC1391990.10307599261801757No Hit
AGGTGGCGGAATTGGTAGACGCG1363100.10093670610968453No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)