FastQCFastQC Report
Tue 18 Jul 2023
SRR10811669.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10811669.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30953910
Total Bases905 Mbp
Sequences flagged as poor quality0
Sequence length18-151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGACTTAGCTCAGTAGGTAGAGCAACTG21949167.090916785633866No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGC16546995.345686538469615No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGC6191402.000199651675669No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAC5859001.8928141872868405No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCC5697911.8407722966177777No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTG4732091.528753556497386No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCT3862161.2477131322020385No Hit
GCGAAGGTGGCGGAATTGGTAGACGCG3761601.2152261216757432No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGG3731911.2056344416585822No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGC2995450.9677129642103371No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAC2853360.9218092318547156No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGT2584980.8351061303725442No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGT2493820.8056558929065828No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTC2106620.6805666876979354TruSeq Adapter, Index 3 (100% over 50bp)
GCGGGCATCGTATAATGGCTATTACCTCAGC2016540.6514653560729485No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGG2007630.6485868828849085No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTT1959330.6329830383302142No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTG1876100.6060946743077046No Hit
TCCTCTGTAGTTCAGTCGGTAGAACG1856140.599646377468953No Hit
GGCGCGTTAACAAAGCGGTTATGTAGCGGATT1646700.5319844891970029No Hit
CGGACGTGGGGGTTCAAGTCCCCCCCCTCGCAC1612970.521087642885826No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGAT1561460.504446772637124No Hit
GCCGAAGTGGCGAAATCGGTAGACGCAGTTGAT1505250.4862875158582551No Hit
CGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGG1342630.43375134191447867No Hit
GATTGTCTGATAGAAAGTGAAAAGC1342380.4336705766735123No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGT1304000.4212714968803618No Hit
TGTGGGCACTCGAAGATACGGATTCTT1262580.4078903117570608No Hit
TGTGGGCACTCGAAGATACGG1236720.39953595523150387No Hit
CCGACTTAGCTCAGTAGGTAGAGCAACTG1235490.3991385902459495No Hit
CCTCTGTAGTTCAGTCGGTAGAACGG1227670.39661225350852286No Hit
GCGAAGGTGGCGGAATTGGTAGACG1219630.3940148433590458No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATT1178860.38084364786225716No Hit
TTCAGGGACGTCATTACGGGCAGGGATGCCCACC1176800.3801781422766946No Hit
CCTCTGTAGTTCAGTCGGTAGAACG1169300.3777551850477048No Hit
GCGGGCGTAGTTCAATGGT1044120.3373144135910455No Hit
GCCCGGATGGTGGAATCGGTAGACAC1042810.33689120372838194No Hit
CGGACGTGGGGGTTCAAGTCCCCCCC1014290.3276775050389434No Hit
GCGGGGTGGAGCAGCCTGGTAGCTCGTCGGG1004900.3246439625882481No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGC966860.3123547235228118No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGG922230.29793651270550314No Hit
AAGGTGGCGGAATTGGTAGACGCG908900.2936301100571786No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGTG887040.28656799738708294No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCT882460.2850883781725798No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCCTT872790.2819643786520023No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGC833320.26921316240823856No Hit
AGGTGGCGGAATTGGTAGACGCG811320.2621058212032018No Hit
TCCCGGTTTAAGCGTGTAGGCT809680.2615760012224627No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCC786080.2539517624752414No Hit
CGGGCATCGTATAATGGCTATTACCTCAGCC784790.25353501383185517No Hit
TGTGGGCACTCGAAGATACGGATTC764930.24711902308949016No Hit
CCTCTGTAGTTCAGTCGGTAGAACGGC713830.2306106078359729No Hit
GGTGAGGTGTCCGAGTGGCTGAAGGAGC712150.2300678654166792No Hit
GTGAGGTGTCCGAGTGGCTGAAGGAGC706310.2281811893877058No Hit
GAAGGTGGCGGAATTGGTAGACGCG704130.22747691648647939No Hit
AGGGGCGTAGTTCAATTGGTAGAGC673770.21766878562352868No Hit
CCTCTGTAGTTCAGTCGGTAGAACGGCGG668260.21588871971263082No Hit
GCCGAAGTGGCGAAATCGGTAGACGCAGTTGATT664710.21474185329090895No Hit
CGGCGGTAACAGGGGTTCGAATCCCCTAGGGGAC654470.21143370902092823No Hit
GCGGGCGTAGTTCAATGGTAGAACGAGAGC653530.2111300317148948No Hit
TGAGGTGTCCGAGTGGCTGAAGGAGC647080.20904628849796358No Hit
GTGGCTATAGCTCAGTTGGTAGAGCCCTGGAT632120.20421329647853856No Hit
CCCGGTTTAAGCGTGTAGGCT607230.19617230908793104No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACG595840.1924926447095052No Hit
GCGAAGGTGGCGGAATTGGTAGACGC585810.18925234324193616No Hit
TAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGC561390.18136319450434532No Hit
TTCCAGTTGTCGTGGGTTCGAATCCCATTAGCC552100.17836195815003664No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCG542330.17520565253307258No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACC539840.17440123073304795No Hit
GATTGTCTGATAGAAAGTG537310.1735838864944687No Hit
TCCCCTTCGTCTAGAGGCCCAGGACACCGCC526470.17008190564616876No Hit
TTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACC525430.16974592224374885No Hit
TTCCGGCATTCCGAGGTTCGAATCCTCGTACCCC520290.16808538888948116No Hit
TAGCTCAGTAGGTAGAGCAACTG499050.16122357401698204No Hit
GCCGAGGTGGTGGAATTGGTAGACACGC493860.15954688761452107No Hit
GCGGGCATCGTATAATGGCTATTACC486740.15724669355180007No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACT485340.15679440820238866No Hit
TGGCTATAGCTCAGTTGGTAGAGCCCTGG482910.15600937006019594No Hit
GAGGTGTCCGAGTGGCTGAAGGAGC482880.15599967823128No Hit
GGCGCGTTAACAAAGCGGTTATGTAGCGGATTG479840.15501757290112944No Hit
TGTGGGCACTCGAAGATACGGAT467040.15088239256365352No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGAC456710.1475451728069249No Hit
CGGACGTGGGGGTTCAAGTCCCCCCCCTCGC442860.14307077845739036No Hit
CGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCT433690.14010830941874547No Hit
CGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACG425070.13732352391022654No Hit
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGT415270.1341575264643465No Hit
TTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGC407850.13176041411246592No Hit
GCGGGCGTAGTTCAATGGTAGAACGAGAGCT403790.13044878659917278No Hit
AGGGGCGTAGTTCAATTGGTAGAGCACC390570.1261779206568734No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTG388370.1254671865363697No Hit
GCCGAGGTGGTGGAATTGGTAGAC383600.1239261857387322No Hit
GTCCCGGTTTAAGCGTGTAGGCT375300.12124477973865014No Hit
CGGAAAATCAAGGCTGAGGCGTG373070.1205243537892305No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCC371700.12008176026873504No Hit
CGGCACGTAGCGCAGCCTGGTAGCGCACCGT362840.1172194401288884No Hit
CGAAGGTGGCGGAATTGGTAGACGCG361170.11667992831923334No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAC359270.11606611248788926No Hit
GCGGGCGTAGTTCAATGGTAGAACG357480.11548783336257035No Hit
TCGTGGAGCTGAAATCAGT354040.11437650364687368No Hit
CTCTGTAGTTCAGTCGGTAGAACGG345650.11166602216004376No Hit
GGGGCGTAGTTCAATTGGTAGAGC340020.10984718893348208No Hit
GCCGAGGTGGTGGAATTGGTAGACACG339390.10964366052624691No Hit
TCCTCTGTAGTTCAGTCGGTAGAAC336550.10872616738886946No Hit
TCGTGGAGCTGAAATCAGTC335190.10828680447801263No Hit
GATTGTCTGATAGAAAGTGAAA332760.10750176633581993No Hit
TGGCTATAGCTCAGTTGGTAGAGCCCTGGAT325060.10501419691405706No Hit
ACGAAGGGGTCGGAGGTTCGAATCCTCTATCACC315490.10192250348986606No Hit
GGAGAGATGCCGGAGCGGCTGAACGGACCGGTC314530.10161236496455536No Hit
TGTGGGCACTCGAAGATACGGATTCTTA314010.10144437326334539No Hit

[OK]Adapter Content

Adapter graph