FastQCFastQC Report
Tue 18 Jul 2023
SRR10811670.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10811670.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18075926
Total Bases508.2 Mbp
Sequences flagged as poor quality0
Sequence length18-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGCACTCGAAGATACGG10467935.791089208929048No Hit
TGTGGGCACTCGAAGATACG6242273.4533611168799876No Hit
TGTGGGCACTCGAAGATACGGATTCT4237232.3441288706315793No Hit
TGTGGGCACTCGAAGATACGGATTCTT4175212.309818041963659No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTG2879921.5932351128235422No Hit
TGTGGGCACTCGAAGATACGGATTC2739511.5155572112875433No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACG2397581.32639401157097No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTG2197941.2159487707573045No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGT2058261.1386747212839883No Hit
GATTGTCTGATAGAAAGTG2025301.1204405240428623No Hit
TGTGGGCACTCGAAGATACGGAT2024221.1198430442788934No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTC1635120.9045843626489729TruSeq Adapter, Index 4 (100% over 50bp)
GATTGTCTGATAGAAAGTGAAAAGC1460120.807770512005858No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAC1432180.79231348922318No Hit
GGGGCTGATTCTGGATTCGACGG1299630.7189839126360663No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGTG1242340.6872898240455289No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGG1029820.56971908382453No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGAT990470.5479497979799209No Hit
CTTTAACAATTTATCAGACAATCTGAA937670.5187396761858839No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGC912920.5050474315949292No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGC892850.493944265981173No Hit
TGTGGGCACTCGAAGATACGGATT846670.46839647385146405No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACG805750.4457586294610854No Hit
TGTGGGCACTCGAAGATACGGATTCTTA719870.3982479237854813No Hit
AGGTGTAGCGGTGAAATGCGTAG709420.3924667538470782No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGC691610.3826138699616274No Hit
TTAACAATTTATCAGACAATCTGAA684570.3787191870557558No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGC660270.3652758923664547No Hit
GATTGTCTGATAGAAAGTGAAA561130.3104294629221208No Hit
ATTTATCAGACAATCTGAA552320.30555557706974457No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGG542010.29985185821185595No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGT539670.2985573187232565No Hit
GATTGTCTGATGAAAATGAGC522670.2891525446607825No Hit
GATTGTCTGATGAAAATG519330.2873047831685082No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAC507530.28077676352514386No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTT483100.267261549975365No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTG477600.2642188289551528No Hit
TGTGGGCACTCGAAGATACGGA475650.2631400460479867No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAC418360.23144595745744923No Hit
TGTGGGCACTCGAAGATAC400640.22164286355232923No Hit
GGGGCTGATTCTGGATTCGACGGG399140.2208130305468168No Hit
GCGAAGGTGGCGGAATTGGTAGACG387440.21434033310381997No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGA382700.21171806080640074No Hit
GCGAAGGTGGCGGAATTGGTAGACGCG371890.2057377309466746No Hit
GGTGGCCGTAGCTCAGTTGGTAGAGTCCCAGATTGTG370850.20516238006285265No Hit
GGTGAGGTGTCCGAGTGGCTGAAGGAGC358660.1984186038380551No Hit
CCTTAAAGAAGCGTACTTTGTAGTGCTCACAC357420.19773260855349817No Hit
GAAGGTGTTTTGGCGGATTGAGAGAAGATTTTC349360.19327363920387813No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTG348490.19279233606068094No Hit
TTTAAAAATCTGGATCAAGCTGAAAATTGAAAC345110.1909224456882596No Hit
GGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGT331820.18357012525941963No Hit
CCGGGGGTAGAGCACTGTTTCGG320910.177534473199326No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGG320270.17718041111697405No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGT320060.1770642344962023No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTG313180.1732580671109187No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTT310960.17202991426276032No Hit
TGTGGGCACTCGAAGATACGGATTCG308550.1706966492339037No Hit
TGGCTATAGCTCAGTTGGTAGAGCCCTGG294740.16305665336315273No Hit
GATTGTCTGATGAAAAGTG283950.15708738794350008No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAA266830.14761622724058507No Hit
CATTTTGTGGCTTAGCTACC266010.1471625851975716No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGC261830.14485011722221036No Hit
GATTGTCTGATAGAAAGT257630.1425265848067756No Hit
TGTGGGCACTCGAAGATACGGATTCTTAA247380.13685605926910743No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCG245280.13569429306139005No Hit
TTTAAAAATCTGGATCAAGCTG244140.13506361997720062No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGC240540.13307202076397082No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGCATGG239880.13270689424154536No Hit
CCTTAAAGAAGCGTACTTTGCAGTGCTCACAC237530.13140682253290925No Hit
TGTGGGCACTCGAAGATACGGATTCTTG228510.12641676005976124No Hit
TTAGTCGCGAGGATGCGC227150.12566437813476333No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGT223740.12377789110223178No Hit
TTTGGCGGATTGAGAGAAGATTTTC223610.12370597224175403No Hit
CTTTAACAATTTATCAGACAATCTGA217560.12035897911952062No Hit
TGTGGGCACTCGAAGATACGGATTCTG213860.11831205770592335No Hit
ACAATTTATCAGACAATCTGAA211560.11703964709747097No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCT205950.11393607165685454No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGC202620.11209384238461696No Hit
ATTGACGGGGGCCCGCACAAGCGGTGGAGC201810.11164573256164027No Hit
TTAACAATTTATCAGACAATCTGA197100.10904005692433129No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCC192130.10629054356606682No Hit
GGGGGCTCTGTTGGTTCTCCCGCAACGCTACTCTGT187680.10382870564971333No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCT185060.10237926400008497No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTC184540.10209158855817399No Hit

[OK]Adapter Content

Adapter graph