FastQCFastQC Report
Tue 18 Jul 2023
SRR10811673.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10811673.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28500608
Total Bases859.3 Mbp
Sequences flagged as poor quality0
Sequence length18-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGACTTAGCTCAGTAGGTAGAGCAACTG660854923.18739656361015No Hit
GCGAAGGTGGCGGAATTGGTAGACGCG14025494.921119577519188No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGC8948463.1397435451201607No Hit
GCGAAGGTGGCGGAATTGGTAGACG8904233.1242245779458457No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTC8676613.044359615065054TruSeq Adapter, Index 6 (100% over 50bp)
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGC3780801.3265681911066598No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGC3676741.2900566893169436No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCT3055321.0720192355194669No Hit
GGCGCGTTAACAAAGCGGTTATGTAGCGGATT2306720.8093581722888158No Hit
GCCCGGATGGTGGAATCGG2257900.7922287131558738No Hit
GCGGGCGTAGTTCAATGGT2113490.7415596186579599No Hit
TGTGGGCACTCGAAGATACGG2052250.7200723577546135No Hit
GCCCGGATGGTGGAATCGGTAGACAC1996710.7005850541855106No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCG1905540.668596262928847No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGG1736250.609197530101814No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGC1602700.5623388806301957No Hit
GCGAAGGTGGCGGAATTGGTAGACGC1447560.5079049541679953No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGC1427630.5009121208922982No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGT1427140.5007401947354948No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGC1389390.4874948632674784No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGTG1386850.4866036542097628No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACT1385330.4860703322539645No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCC1362080.47791261154849746No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGAT1352690.4746179449926121No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGG1326220.4653304238281513No Hit
GCCGAAGTGGCGAAATCGGTAGACGCAGTTGATT1217250.4270961517733236No Hit
GCCGAGGTGGTGGAATTGGTAGACACG1156000.4056053821728996No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCG1123040.39404071660506335No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAT1082780.3799147021705642No Hit
GCGGGCGTAGTTCAATGGTAGAACGAGAGC1044540.36649744454574446No Hit
TGTGGGCACTCGAAGATACGGATTCTT978600.3433610960159166No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGT936540.32860351610744587No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTG862950.3027830143132385No Hit
GGCGCGTTAACAAAGCGGTTATGTAGCGGATTG862790.3027268751599966No Hit
GCCGAGGTGGTGGAATTGGTAGACACGC767630.2693381137693624No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCAC746370.2618786237823418No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACC714520.2507034235901213No Hit
GGTGAGGTGTCCGAGTGGCTGAAGGAGC690630.24232114627168655No Hit
CAGTTCGACTCTGGGTATCAGCACC684590.2402018932368039No Hit
TAGCTCAGTAGGTAGAGCAACTG638160.22391101270541314No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGT626870.2199496937047799No Hit
TCCTCTGTAGTTCAGTCGGTAGAACG622750.2185041105088004No Hit
GGAGAGATGCCGGAGCGGCTGAACGGACCGGT572520.2008799250879139No Hit
GCGAAGGTGGCGGAATTGG518260.1818417347447465No Hit
TTCAGGGACGTCATTACGGGCAGGGATGCCCACC489890.17188756113553788No Hit
TGTGGGCACTCGAAGATACGGATTC486680.1707612693736218No Hit
TGGGGTATCGCCAAGCGG469200.1646280668819416No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTT458930.1610246349832256No Hit
GGTGAGGTGTCCGAGTGGCTGAAGGAGCACG425380.1492529562878097No Hit
AGGGGCGTAGTTCAATTGGTAGAGC424390.14890559527712532No Hit
AGGCTTGTAGCTCAGGTGGTT418150.1467161683006903No Hit
CGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGG413790.14518637637484785No Hit
GCGAAGGTGGCGGAATTGGT409870.14381096712042074No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGC407000.14280397105914372No Hit
TGTGGGCACTCGAAGATACGGATTCT401950.1410320790349455No Hit
CGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTC398380.13977947417823508No Hit
GCCCTGGATTGTGATTCC385760.13535149846627834No Hit
GGTGGCTATAGCTCAGTTGG373650.13110246630528022No Hit
TGTGGGCACTCGAAGATACG373000.1308744009952349No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGG367860.1290709306973381No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAC367080.12879725232528372No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCCTGCT353370.12398682863186637No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAC332400.11662909086009673No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCT331570.11633786900265425No Hit
GCCGAGGTGGTGGAATTGGTAGAC330510.11596594711242653No Hit
GTTCAAGTCCCCCCCCTCGCACC324870.1139870419606487No Hit
ACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTT324770.1139519549898725TruSeq Adapter, Index 6 (100% over 50bp)
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGCATGG322870.11328530254512464No Hit
TTTCTCTGAGATGTTCGC320890.11259058052375584No Hit
TCCCGGTTTAAGCGTGTAGGCT316240.11095903638266244No Hit
GCGGGCGTAGTTCAATGGTAGAACG310360.10889592250102173No Hit
GATTGTCTGATAGAAAGTGAAAAGC301710.10586089952888023No Hit
GCCGAGGTGGTGGAATTGGTAGACACGCT297860.10451005115399645No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTG295300.10361182470212564No Hit
GGGTTCAAGTCCCCCCCCTCGCACC289750.10166449782404642No Hit
GCCGAAGTGGCGAAATCGGTAGACGC285360.10012417980697114No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)