FastQCFastQC Report
Wed 30 Aug 2023
SRR10850867_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10850867_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78068579
Total Bases7.8 Gbp
Sequences flagged as poor quality0
Sequence length87-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC894215211.45422667421678No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC34166784.376508505425723No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG33451594.284898025363059No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAAG32217094.126767825503779No Hit
TTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAA30495583.9062552938231394No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG22691592.9066226503238903No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA16363392.0960276476916535No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGTGGAATTCTCGGGTGCCA13252281.6975177683200817No Hit
TTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCAA12066991.5456910007289872No Hit
TCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCAAG11920851.5269715617598216No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA8666271.1100842504127044No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA8325951.0664918084393467No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG7557180.9680181318530212Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG7522820.963616873313398No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA7368430.9438406711616976No Hit
TTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCA7067910.9053463109659009No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA6428530.8234465238569284No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA6333660.8112943877203145No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA6108260.7824223366483973No Hit
CCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT5132270.6574053307669402RNA PCR Primer, Index 1 (100% over 30bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC5032680.6446485979974094No Hit
ACCCCCCTCGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG4911240.629093043950499RNA PCR Primer, Index 10 (100% over 36bp)
CCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC4607970.5902464293605242RNA PCR Primer, Index 1 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA4437360.568392566745707No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCTGGAATTCTCGGGTGCC4200850.5380974079213098No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCAA4129580.5289682549492799No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG3804530.4873317855574135Illumina Small RNA Adapter 2 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA3435570.4400707741843233No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC3192700.408960947015572RNA PCR Primer, Index 1 (100% over 24bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCTGGAATTCTCGGGTGCCAA3123080.4000431466800491No Hit
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC3037190.3890412812560608RNA PCR Primer, Index 1 (100% over 24bp)
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC2786190.35689006200561174No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCC2627130.3365156678463431No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG2466580.31595041585168343No Hit
CGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGAA2261310.2896568669451509RNA PCR Primer, Index 1 (100% over 23bp)
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA2138240.2738925221118729RNA PCR Primer, Index 1 (100% over 22bp)
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG2125000.2721965773195385No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG1963070.2514545576652548No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG1914420.24522285720097453No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG1882840.24117769583073875Illumina Small RNA Adapter 2 (100% over 21bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCA1822850.23349342633737447No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1805580.23128126874193525No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGA1773370.2271554090923059RNA PCR Primer, Index 1 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCT1675390.2146049052590031No Hit
CATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG1612080.2064953686424855Illumina Small RNA Adapter 2 (100% over 21bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCG1508780.19326341267208155RNA PCR Primer, Index 10 (100% over 45bp)
TTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGAA1458620.18683829252227072RNA PCR Primer, Index 1 (100% over 23bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG1420530.18195924893163484No Hit
CGCAGAAGGTCCTGGGTTCGAGCCCCAGTGGAACCTGGAATTCTCGGGTG1367350.1751472894107628No Hit
AGCAGAGTCGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1354360.17348336774517184No Hit
TCCCTGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACT1310020.1678037459859491RNA PCR Primer, Index 1 (100% over 25bp)
GTTTCCGTAGTGTAGTGGTCATCACTTTCGCCTTGGAATTCTCGGGTGCC1302770.1668750753103883No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCTGGAATTCTCGGGTGCCAA1301780.16674826372848414No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAA1215070.15564136244877724RNA PCR Primer, Index 1 (100% over 23bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCA1180960.1512721270358975No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG1159520.14852582368637707Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG1119050.14334191992914333Illumina Small RNA Adapter 2 (100% over 21bp)
CCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAAC1077210.1379825294373553RNA PCR Primer, Index 1 (100% over 24bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA1076770.1379261687342868No Hit
TTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCAAG1074160.13759184729108492No Hit
TTCCATGGTGTAATGGTTAGCACTCTGGACTTGGAATTCTCGGGTGCCAA1051700.13471488958445113No Hit
AGCAGAGTAGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1000210.12811940640036498No Hit
ATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGA977290.1251835261405232RNA PCR Primer, Index 1 (100% over 22bp)
TCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCAAGG971020.12438038612179683Illumina Small RNA Adapter 2 (100% over 21bp)
TTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCCAA959510.12290604136652725No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGA954810.12230400658374992RNA PCR Primer, Index 1 (100% over 22bp)
TCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCCAAG947870.12141504458535103No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGTGGAATTCTCGGGTGCCAAGG946820.12128054745302844Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTTGGAATTCTCGGGTGCC923010.11823066486197986No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCGTGGAATTCTCGGGTGCC884040.11323889986520697No Hit
CCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAAGG874270.11198743607207197Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATACTCGGGTGCC867570.11112921627534683No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTGGAATTCTCGGGTGCCA860860.11026971555355197No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTTGGAATTCTCGGGTGCCA859070.11004042996606868No Hit
ACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC859050.11003786811592921RNA PCR Primer, Index 10 (100% over 44bp)
GTTTCCGTAGTGTAGTGGTTATCACATTCGCCTTGGAATTCTCGGGTGCC854790.10949219403622039No Hit
CATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG840070.10760667233356458Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGTC796260.101994939603038No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)