FastQCFastQC Report
Tue 25 Jul 2023
SRR10850868_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10850868_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68411183
Total Bases6.8 Gbp
Sequences flagged as poor quality0
Sequence length90-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC41375486.048057961517783No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAAG40531475.924684857445017No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG27273843.986751698183614No Hit
TTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAA23433073.425327405900874No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG17716412.5896950210611034No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG15419822.2539911347535093No Hit
TCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCAAG14712522.1506016055883728No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC12664361.851211957553782No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGTGGAATTCTCGGGTGCCA10119351.4791952947225018No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA9821321.4356307798390213No Hit
TTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCAA9228361.3489548923602153No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG9126831.334113751548486Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC9082241.3275958113456392RNA PCR Primer, Index 1 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA8704011.272308066942798No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG7166911.047622579483825Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA6998691.0230330324794998No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA6775380.9903907084898679No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA5041060.7368766010083468RNA PCR Primer, Index 1 (100% over 22bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCAA4755700.6951641225090348No Hit
TTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCA4301480.6287685450491333No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA4111420.6009865375372913No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG4064130.5940739250189548No Hit
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC3682550.538296494595043RNA PCR Primer, Index 1 (100% over 24bp)
TCCCTGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACT3648440.5333104676760231RNA PCR Primer, Index 1 (100% over 25bp)
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCTGGAATTCTCGGGTGCC3327320.48637077362044745No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC3193000.4667365567994929No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG3165580.4627284401732974No Hit
CGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGAA3163950.462490175034687RNA PCR Primer, Index 1 (100% over 23bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA2957470.43230797514494085No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCC2653040.3878079406988182No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGAA2596590.3795563658064501RNA PCR Primer, Index 1 (100% over 23bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA2573500.3761811866343548No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCA2485400.3633031751548573No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGA2364240.34559262043458594RNA PCR Primer, Index 1 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG2324490.33978216690098756Illumina Small RNA Adapter 2 (100% over 21bp)
ACCCCCCTCGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA2117260.3094903358124943RNA PCR Primer, Index 4 (100% over 36bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG1960760.28661395900725767Illumina Small RNA Adapter 2 (100% over 21bp)
CATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG1825470.2668379525025901Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1793400.26215012244416236No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGGAATTCTCGGGTGCCAA1749800.25577689542366194No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG1746740.2553296001327736Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG1715080.25070170179632767No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTTGGAATTCTCGGGTGCCA1675730.24494971823539438No Hit
TGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGAA1559830.22800804365566957RNA PCR Primer, Index 1 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA1529640.223595022468768No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCTGGAATTCTCGGGTGCCAA1500770.21937495219166142No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1479560.21627458189109228No Hit
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC1460850.21353964891968027No Hit
CCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1446690.21146981188733424RNA PCR Primer, Index 1 (100% over 31bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAA1413990.20668989162195894RNA PCR Primer, Index 1 (100% over 23bp)
CCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAAC1379510.20164977997822373RNA PCR Primer, Index 1 (100% over 24bp)
TCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCCAAG1337770.1955484383306162No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1320530.1930283825087486No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCA1269450.18556176699941002No Hit
ATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGA1186420.17342486242344324RNA PCR Primer, Index 1 (100% over 22bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG1142960.16707209989337563No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC1136560.16613658033073336No Hit
AGCAGAGTCGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1112100.16256114150225995No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG1091030.1594812356921236No Hit
CCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACT1055440.15427886987424264RNA PCR Primer, Index 1 (100% over 25bp)
CGCAGAAGGTCCTGGGTTCGAGCCCCAGTGGAACCTGGAATTCTCGGGTG1034930.15128082202583748No Hit
CCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAAGG1031290.1507487452745847Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC1022970.14953256984314975No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA1011050.14779016465772854No Hit
CCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT987300.14431851003073579RNA PCR Primer, Index 1 (100% over 30bp)
CATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG967250.14138770265089554Illumina Small RNA Adapter 2 (100% over 21bp)
ATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGA939460.13732550129998483RNA PCR Primer, Index 1 (100% over 22bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCTGGAATTCTCGGGTGCCAA896810.1310911404645641No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAAT896650.13106775247549804No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTGC870490.12724381626319778No Hit
TTCCATGGTGTAATGGTTAGCACTCTGGACTTGGAATTCTCGGGTGCCAA856930.12526168418984948No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGTGGAATTCTCGGGTGCCAAGG836290.12224463360032817Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGAGTAGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA833430.12182657329577241No Hit
TTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCCAA823180.12032828024622817No Hit
CGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAACT821250.12004616262811885RNA PCR Primer, Index 1 (100% over 25bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC804280.11756557403780023No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA788530.11526331886411026No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA775940.11342297647447494RNA PCR Primer, Index 1 (100% over 22bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTGCCA738950.10801596575226596No Hit
TCCCATGGTGTAATGGTTAGCACTCTGGACTTGGAATTCTCGGGTGCCAA737940.10786832907128648No Hit
TCCATGGTGTAATGGTTAGCACTCTGGACTTGGAATTCTCGGGTGCCAAG725810.10609522715021606No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGTGGAATTCTCGGGTGCCAA705700.10315564927447607No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)