FastQCFastQC Report
Thu 24 Aug 2023
SRR10850869_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10850869_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53159052
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length91-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1009247618.98543262208664No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA26630265.0095438120303575No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA21678174.077982805261463No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC18400663.461434940562898No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG18337323.449519754415485No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA16121053.0326067515274726No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA14177862.6670641154398314No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG12489902.3495339984618235No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA11107572.089497382308473No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA8977271.688756601603806No Hit
TTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAA8109861.5255840153056153No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC7634741.4362069511698592No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA7597091.4291244320910765No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG6332491.1912345615192685No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG5146040.9680458560472447Illumina Small RNA Adapter 2 (100% over 21bp)
TCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAAG4330540.8146383046860957No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA4168140.7840884747154633No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA4031640.7584108159039404No Hit
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC2763470.5198493757939852No Hit
CCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT2582780.4858589276573254RNA PCR Primer, Index 1 (100% over 30bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG2553590.48036785908070745No Hit
TTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCA2442270.4594269288323652No Hit
CGCGGGCCGGTCCCCCCCGCCGGGTCCGCCCCCGGGGCTGGAATTCTCGG2132150.4010887929303179No Hit
TTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCAA2055360.3866434638450663No Hit
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG1988600.3740849253669911No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCTGGAATTCTCGGGTGCCAA1976760.3718576471228268No Hit
CCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1903140.3580086416890956RNA PCR Primer, Index 1 (100% over 31bp)
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCTGGAATTCTCGGGTGCC1883720.35435545389334633No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCAA1779270.33470687174782576No Hit
ACCCCCCTCGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACA1724380.3243812549554119RNA PCR Primer, Index 5 (100% over 36bp)
CCGCAGCAACCTCGGTTCGAATCCGAGTCACGGCTGGAATTCTCGGGTGC1662120.31266923270189245No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1616290.3040479352415841No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCT1546220.290866737051669No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTGGAATTCTCGGGTGCCA1502350.2826141444358338No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGTGGAATTCTCGGGTGCCA1345450.25309894540632516No Hit
TTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCAAG1340010.25207560134819557No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA1309100.2462609754590808No Hit
AGCAGAGTCGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1209290.22748524559843544No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA1203630.2264205163026609No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA1167120.21955244800076573No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC1160660.21833722693173685No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCC1121100.21089540874430945No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCGTGGAATTCTCGGGTGCC1120400.21076372844271188No Hit
TCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCAAG1108880.2085966469078493No Hit
CCCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG1065400.20041741903147559RNA PCR Primer, Index 1 (100% over 29bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG1055710.19859458742793232Illumina Small RNA Adapter 2 (100% over 21bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG1054090.19828984158709226No Hit
GTTTCCGTAGTGTAGTGGTTATCACATTCGCCTTGGAATTCTCGGGTGCC1003910.18885024510971338No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATACTCGGGTGCC1000700.18824639686953032No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA957480.1801160788194643No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA939100.17665853032894566No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCAA926290.17424878080971046No Hit
AGCAGAGTAGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA897070.16875206879159546No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG827730.15570819434477498No Hit
TGCAACTGCCGTCAGCCATTGATGATCGTTGGAATTCTCGGGTGCCAAGG806990.1518066951231561Illumina Small RNA Adapter 2 (100% over 21bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG806860.15178224021000225RNA PCR Primer, Index 5 (100% over 45bp)
GTTTCCGTAGTGTAGTGGTTATCACCTTCGCCTTGGAATTCTCGGGTGCC784690.14761173694369117No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG766870.1442595326944506Illumina Small RNA Adapter 2 (100% over 21bp)
AGTAAGGTCAGCTAAATAAGCTATCGGGCTGGAATTCTCGGGTGCCAAGG760470.14305559850841584Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTTGGAATTCTCGGGTGCC758250.14263798383763504No Hit
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTGGAATTCTCGGGTGCCA753630.14176889384709118No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTATCGGGTGCC745410.14022259087690278No Hit
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC729720.1372710709739519RNA PCR Primer, Index 1 (100% over 24bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGTC726430.1366521735564434No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG708790.13333382995618506No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA666530.12538410203402425No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCA665960.12527687664558051No Hit
GTTTCCGTAGTGTAGTGGTCATCACTTTCGCCTTGGAATTCTCGGGTGCC647740.12184942650971277No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA612680.11525412454684106No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGA577950.10872090044043675RNA PCR Primer, Index 1 (100% over 22bp)
TCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCAAGG575640.10828635544516484Illumina Small RNA Adapter 2 (100% over 21bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCTGGAATTCTCGGGTGCCAA569860.10719905238340219No Hit
CGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGAA567700.10679272459561542RNA PCR Primer, Index 1 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA540260.10163085677299137No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)