FastQCFastQC Report
Thu 24 Aug 2023
SRR10850870_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10850870_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68600700
Total Bases6.8 Gbp
Sequences flagged as poor quality0
Sequence length90-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1164273716.971746643984684No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC25671933.7422256624203545No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG23237743.387391090761464No Hit
TTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAA19547072.849398038212438No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG18553082.704503015275354No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA16804442.449601826220432No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA16784932.446757831917167No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA16254562.3694452097427576No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA14103442.0558740654249883No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAAG12498091.8218604183339235No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA10290481.5000546641652344No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA8510601.2405995857185133No Hit
CCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT8093451.1797911683116935RNA PCR Primer, Index 1 (100% over 30bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA8088031.1790010889101714No Hit
ACCCCCCTCGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC7029591.0247111181081243RNA PCR Primer, Index 6 (100% over 36bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC6906311.006740456001178No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG6359240.9269934563349936Illumina Small RNA Adapter 2 (100% over 21bp)
TTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCAA5420790.7901945606969026No Hit
TTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCA5040580.7347709279934461No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG4810880.7012873046484948No Hit
CCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC4782910.6972100867775402RNA PCR Primer, Index 1 (100% over 31bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG4187080.6103552879198026No Hit
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC3930010.5728819093682718No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA3489100.5086099704521965No Hit
TCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCAAG3375160.49200081048735655No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGTGGAATTCTCGGGTGCCA3251990.4740461832022122No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA3027900.4413803357691685No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCAA2357680.3436816242399859No Hit
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG2259540.32937564777035805No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCTGGAATTCTCGGGTGCCAA2250760.32809577744833507No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCC2021490.2946748356795193No Hit
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC2013220.2934693086222152RNA PCR Primer, Index 1 (100% over 24bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCT1982480.28898830478406196No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCTGGAATTCTCGGGTGCC1933120.2817930429281334No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1894980.27623333289602003No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG1832770.2671649123113904Illumina Small RNA Adapter 2 (100% over 21bp)
TTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCAAG1689010.24620885792710573No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCAA1645100.23980804860591803No Hit
CCCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG1643410.2395616954345947RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCGTGGAATTCTCGGGTGCC1419020.20685211666936343No Hit
CGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGAA1375650.2005300237461134RNA PCR Primer, Index 1 (100% over 23bp)
AGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1361040.1984003078685786RNA PCR Primer, Index 1 (100% over 33bp)
AGCAGAGTCGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1360730.1983551188253181No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG1298100.1892254743756259Illumina Small RNA Adapter 2 (100% over 21bp)
AGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1281420.18679401230599688RNA PCR Primer, Index 1 (100% over 32bp)
TCCCTGGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC1215820.17723142766764768RNA PCR Primer, Index 1 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG1099640.16029574042247383No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGA1099360.1602549245124321RNA PCR Primer, Index 1 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTTATCACATTCGCCTTGGAATTCTCGGGTGCC1088580.1586835119758253No Hit
GTTTCCGTAGTGTAGTGGTCATCACTTTCGCCTTGGAATTCTCGGGTGCC1060960.1546573139924228No Hit
AGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1058670.15432349815672433RNA PCR Primer, Index 1 (100% over 31bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCA1054780.15375644854935883No Hit
TGCAACTGCCGTCAGCCATTGATGATCGTTGGAATTCTCGGGTGCCAAGG1052920.15348531428979587Illumina Small RNA Adapter 2 (100% over 21bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG1044000.15218503601275207No Hit
CACCCCCCTCGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC1043800.15215588179129366RNA PCR Primer, Index 6 (100% over 35bp)
TTCCATGGTGTAATGGTTAGCACTCTGGACTTGGAATTCTCGGGTGCCAA1036810.15113694175132322No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTGGAATTCTCGGGTGCCA1034720.15083228013708316No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA980370.14290962045576797No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAA957100.13951752678908524RNA PCR Primer, Index 1 (100% over 23bp)
AGCAGAGTAGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA953600.13900732791356357No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCTGGAATTCTCGGGTGCCAA940940.13716186569524802No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG940140.1370452488094145No Hit
AATCTCTGCAACTCTCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA936940.13657878126608036RNA PCR Primer, Index 1 (100% over 32bp)
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA920520.13418521968434724RNA PCR Primer, Index 1 (100% over 22bp)
TCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCAAGG920100.13412399581928464Illumina Small RNA Adapter 2 (100% over 21bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA918100.13383245360470084No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTATCGGGTGCC915320.1334272099264293No Hit
GTTTCCGTAGTGTAGTGGTTATCACCTTCGCCTTGGAATTCTCGGGTGCC907020.13221730973590648No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA894230.130352897273643No Hit
CCGCAGCAACCTCGGTTCGAATCCGAGTCACGGCTGGAATTCTCGGGTGC852550.12427715752171625No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGTC840820.12256726243318217No Hit
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCA780620.1137918417742093RNA PCR Primer, Index 1 (100% over 28bp)
GAATACAAGCTTGGGCTGCAGGTCTGGAATTCTCGGGTGCCAAGGAACTC751850.10959800701742112RNA PCR Primer, Index 1 (100% over 26bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGATGGAATTCTCGGGTGCC747190.10891871365744082No Hit
CGGGGCACCCCCCTCGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT725390.10574090351847723RNA PCR Primer, Index 1 (100% over 30bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG712200.10381818261329695RNA PCR Primer, Index 6 (100% over 45bp)
CCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAAC702870.10245813818226343RNA PCR Primer, Index 1 (100% over 24bp)
CGGCGGCGGCCCGCCGCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCA694910.10129780016821985RNA PCR Primer, Index 1 (100% over 28bp)
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTTGGAATTCTCGGGTGCC689630.10052812872171858No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)