FastQCFastQC Report
Wed 30 Aug 2023
SRR10850872_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10850872_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70739023
Total Bases7 Gbp
Sequences flagged as poor quality0
Sequence length88-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAAG40268425.6925326774728005No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC36043445.09526969293879No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG25305173.577257491950377No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG17674272.498517685210326No Hit
TTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAA17416872.462130414212817No Hit
TCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCAAG17299832.4455850909900185No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG15070312.1304096891471063No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC13576761.9192744576073661No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGTGGAATTCTCGGGTGCCA11454751.619297173499272No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG10468271.4798437349071107Illumina Small RNA Adapter 2 (100% over 21bp)
TTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCAA8083621.1427384288301523No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA7303911.0325149670218092No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA6633610.9377582158577452No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA6522030.9219847438379237No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA5878140.8309614341153679RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG5688700.8041813073952124Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA5558890.7858307570914571No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC5346040.7557412829973634RNA PCR Primer, Index 1 (100% over 24bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCAA5075440.7174879981025466No Hit
CGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGAA4591270.6490434565374192RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG4182500.5912578125372187No Hit
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC4127600.5834968911006871RNA PCR Primer, Index 1 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA3678430.5200001136572101No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGA3543260.5008918486193964RNA PCR Primer, Index 1 (100% over 22bp)
TTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCA3395620.4800207659073833No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG3283880.4642246755372915Illumina Small RNA Adapter 2 (100% over 21bp)
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCTGGAATTCTCGGGTGCC3183920.45009386120585804No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC3051570.4313842445915601No Hit
ACCCCCCTCGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT3033470.4288255437172209RNA PCR Primer, Index 12 (100% over 36bp)
TTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGAA2906370.41085809172117066RNA PCR Primer, Index 1 (100% over 23bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG2826950.39963090810570007No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCC2571800.36356170765886886No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA2376790.33599417961992495No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCA2346840.3317603071786841No Hit
CATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG2204390.3116229072035671Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACT2180420.30823439560368254RNA PCR Primer, Index 1 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG2089030.2953150766586075Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG1811070.2560213476513522Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA1808480.2556552131063501No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1764340.249415375725503No Hit
CCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAAGG1617720.2286884849964637Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGAA1612690.22797742060983792RNA PCR Primer, Index 1 (100% over 23bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGGAATTCTCGGGTGCCAA1367880.1933699310492315No Hit
CCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1365900.19309002896463526RNA PCR Primer, Index 1 (100% over 31bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA1332740.1884023758710945No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAAC1324060.18717533036892522RNA PCR Primer, Index 1 (100% over 24bp)
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG1303170.1842222220117459No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAA1297530.18342492516471423RNA PCR Primer, Index 1 (100% over 23bp)
TCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCCAAG1256320.1775992863231939No Hit
ATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGA1200510.16970972301949944RNA PCR Primer, Index 1 (100% over 22bp)
CCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACT1193570.16872865207652077RNA PCR Primer, Index 1 (100% over 25bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG1191820.16848126387043824No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1191140.16838513588178905No Hit
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC1168360.16516484826204061No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1153410.16305144615864994No Hit
CGCAGAAGGTCCTGGGTTCGAGCCCCAGTGGAACCTGGAATTCTCGGGTG1133910.16029483471944472No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC1127900.15944523293741278No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGA1109760.156880877475506RNA PCR Primer, Index 1 (100% over 22bp)
CCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1101900.1557697510184725RNA PCR Primer, Index 1 (100% over 30bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGACTTGGAATTCTCGGGTGCCA1082910.1530852355707542No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCTGGAATTCTCGGGTGCCAA1076030.15211264650912693No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC1060120.14986353430411387No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCA1057040.14942813106140865No Hit
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG1008760.14260304386731493RNA PCR Primer, Index 12 (100% over 45bp)
CATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG1003660.14188208395244586Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC968010.13684243278282202No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAAT955330.13504992852389266No Hit
AGCAGAGTCGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA871240.12316257180990468No Hit
CCCAGGTGGCCCGGGTTCGACTCCCGGTATGGGATGGAATTCTCGGGTGC870690.12308482123085018No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG857900.12127676685610995No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA853690.12068162151462002No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA836070.11819077569109203RNA PCR Primer, Index 1 (100% over 22bp)
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA831870.11759704399649398No Hit
CCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCAAGG793800.1122152902790303Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGTGGAATTCTCGGGTGCCAAGG751590.10624828674831995Illumina Small RNA Adapter 2 (100% over 21bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCTGGAATTCTCGGGTGCCAA743620.10512161017547557No Hit
TCCCATGGTGTAATGGTTAGCACTCTGGACTTGGAATTCTCGGGTGCCAA713780.10090328785004567No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)