FastQCFastQC Report
Thu 24 Aug 2023
SRR10850873_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10850873_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78868784
Total Bases7.9 Gbp
Sequences flagged as poor quality0
Sequence length88-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC902848011.447469508341856No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC34502364.374653475068159No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG33716064.274956236170701No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAAG32574314.130190469273623No Hit
TTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAA30818063.907510479684839No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG22900542.9036253430761656No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA16546352.0979593142960082No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGTGGAATTCTCGGGTGCCA13359841.693932544972419No Hit
TTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCAA12183341.544760725612303No Hit
TCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCAAG12060491.5291842207177937No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA8752541.109759724455749No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA8402081.0653238929105335No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG7669760.9724709334937888Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG7633780.9679089257924909No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA7440130.9433554852322815No Hit
TTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCA7119250.9026701869779049No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA6464070.8195980300647212No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA6404020.8119841178228384No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA6183270.7839945903058427No Hit
CCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT5184340.6573373820496586RNA PCR Primer, Index 1 (100% over 30bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC5101540.6468389318643483No Hit
ACCCCCCTCGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG4961980.6291437180012818RNA PCR Primer, Index 10 (100% over 36bp)
CCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC4666530.5916827625997124RNA PCR Primer, Index 1 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA4476750.5676200104720773No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCTGGAATTCTCGGGTGCC4248120.5386313550872041No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCAA4185030.5306319925003535No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG3846010.4876466714638329Illumina Small RNA Adapter 2 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA3466400.43951482756473076No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC3212590.4073335275462089RNA PCR Primer, Index 1 (100% over 24bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCTGGAATTCTCGGGTGCCAA3147600.3990932584937533No Hit
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC3063610.38844392478524836RNA PCR Primer, Index 1 (100% over 24bp)
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC2797300.35467771380879914No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCC2653800.33648293601179396No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG2503590.3174373780125734No Hit
CGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGAA2279860.2890700077232077RNA PCR Primer, Index 1 (100% over 23bp)
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA2154140.27312960727275826RNA PCR Primer, Index 1 (100% over 22bp)
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG2132950.27044286621687996No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG1997040.2532104463535282No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG1930420.24476350491215892No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG1918610.2432660810391092Illumina Small RNA Adapter 2 (100% over 21bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCA1838230.23307446961525363No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1822700.23110537624112473No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGA1784880.22631006964681996RNA PCR Primer, Index 1 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCT1641930.20818502793196356No Hit
CATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG1633890.20716561320382473Illumina Small RNA Adapter 2 (100% over 21bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCG1524370.19327925735484905RNA PCR Primer, Index 10 (100% over 45bp)
TTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGAA1478850.18750764561046104RNA PCR Primer, Index 1 (100% over 23bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG1429120.18120223585544312No Hit
CGCAGAAGGTCCTGGGTTCGAGCCCCAGTGGAACCTGGAATTCTCGGGTG1386130.17575141008893963No Hit
AGCAGAGTCGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1378330.174762425651193No Hit
TCCCTGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACT1325580.16807410140873988RNA PCR Primer, Index 1 (100% over 25bp)
GTTTCCGTAGTGTAGTGGTCATCACTTTCGCCTTGGAATTCTCGGGTGCC1321610.16757073368850217No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCTGGAATTCTCGGGTGCCAA1319480.16730066486127135No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAA1233070.15634449239131162RNA PCR Primer, Index 1 (100% over 23bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCA1192870.15124741875061748No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG1176230.1491375852834247Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG1129830.1432543958075986Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA1099030.13934917520726578No Hit
TTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCAAG1090260.138237201679184No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAAC1077870.13666623793768648RNA PCR Primer, Index 1 (100% over 24bp)
TTCCATGGTGTAATGGTTAGCACTCTGGACTTGGAATTCTCGGGTGCCAA1070480.13572923857935987No Hit
AGCAGAGTAGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1010980.128185062419626No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGA980830.12436225718910539RNA PCR Primer, Index 1 (100% over 22bp)
ATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGA980630.12433689861377854RNA PCR Primer, Index 1 (100% over 22bp)
TCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCAAGG976480.12381070817574669Illumina Small RNA Adapter 2 (100% over 21bp)
TTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCCAA969060.12286990503112105No Hit
TCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCCAAG962520.1220406796179335No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGTGGAATTCTCGGGTGCCAAGG955810.12118989941571814Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTTGGAATTCTCGGGTGCC931610.11812151180117092No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATACTCGGGTGCC905310.11478685914569191No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCGTGGAATTCTCGGGTGCC902980.11449143174313427No Hit
CCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAAGG882280.11186681919680669Illumina Small RNA Adapter 2 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGACTTGGAATTCTCGGGTGCCA879750.1115460332189222No Hit
GTTTCCGTAGTGTAGTGGTTATCACATTCGCCTTGGAATTCTCGGGTGCC866140.10982038216793097No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTGGAATTCTCGGGTGCCA863600.10949832826128016No Hit
ACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC862150.10931447859016058RNA PCR Primer, Index 10 (100% over 44bp)
CATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG860310.10908117969715368Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGTC800430.10148882224429884No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)