FastQCFastQC Report
Wed 30 Aug 2023
SRR10850874_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10850874_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68880304
Total Bases6.9 Gbp
Sequences flagged as poor quality0
Sequence length90-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC41651956.047004380236184No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAAG40948845.944927304618168No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG27465913.98748385314908No Hit
TTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAA23641493.4322569191912975No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG17816122.586533299853032No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG15556162.258433702615482No Hit
TCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCAAG14851272.1560982076966444No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC12781871.8556639935851618No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGTGGAATTCTCGGGTGCCA10201361.4810271452925061No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA9888431.4355961611319252No Hit
TTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCAA9297761.34984305528036No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG9227571.3396529144238387Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC9092921.3201045105724274RNA PCR Primer, Index 1 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA8791201.276300987289487No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG7236281.0505586618781473Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA7046421.0229949043198183No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA6837020.9925943416277606No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA5091020.7391111398114619RNA PCR Primer, Index 1 (100% over 22bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCAA4810480.6983825158495235No Hit
TTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCA4335220.629384562530386No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA4126560.5990914325813661No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG4106570.5961892967255197No Hit
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC3719610.5400106828796807RNA PCR Primer, Index 1 (100% over 24bp)
TCCCTGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACT3677750.5339334739289188RNA PCR Primer, Index 1 (100% over 25bp)
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCTGGAATTCTCGGGTGCC3367650.4889133474207663No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC3227700.4685954928421918No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG3202610.4649529421356793No Hit
CGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGAA3195680.4639468490150682RNA PCR Primer, Index 1 (100% over 23bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA2976080.4320654566216781No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCC2675240.3883896911953234No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGAA2610070.3789283508388697RNA PCR Primer, Index 1 (100% over 23bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA2592210.37633544706771327No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCA2519350.365757677259961No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGA2372260.3444032418904539RNA PCR Primer, Index 1 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG2351810.3414343235186651Illumina Small RNA Adapter 2 (100% over 21bp)
ACCCCCCTCGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA2128680.309040447905108RNA PCR Primer, Index 4 (100% over 36bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG1989000.2887617917598041Illumina Small RNA Adapter 2 (100% over 21bp)
CATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG1844030.26771513668116215Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1805410.26210830892964704No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGGAATTCTCGGGTGCCAA1772420.2573188411015143No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG1764540.25617482756754384Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG1727550.25080464220947685No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTTGGAATTCTCGGGTGCCA1690210.24538364406754068No Hit
TGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGAA1568330.2276891809304442RNA PCR Primer, Index 1 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA1559270.22637385572514315No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCTGGAATTCTCGGGTGCCAA1525710.2215016356489948No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1494960.2170373696376253No Hit
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC1470550.21349354091120157No Hit
CCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1451130.21067415730337077RNA PCR Primer, Index 1 (100% over 31bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAA1417380.2057743531445506RNA PCR Primer, Index 1 (100% over 23bp)
CCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAAC1388460.20157576540312597RNA PCR Primer, Index 1 (100% over 24bp)
TCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCCAAG1351780.1962505856536289No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1324640.19231041721302508No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCA1277930.1855290882572179No Hit
ATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGA1205230.174974547150663RNA PCR Primer, Index 1 (100% over 22bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG1148950.16680385150448812No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC1140080.16551611038185893No Hit
AGCAGAGTCGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1128000.16376234344145751No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG1095620.1590614350366398No Hit
CCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACT1064810.15458845826232126RNA PCR Primer, Index 1 (100% over 25bp)
CGCAGAAGGTCCTGGGTTCGAGCCCCAGTGGAACCTGGAATTCTCGGGTG1046920.15199119910969033No Hit
CCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAAGG1033780.15008354202385635Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC1028720.14934893434848953No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA1015600.1474441808503052No Hit
CCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT996910.14473077819168742RNA PCR Primer, Index 1 (100% over 30bp)
CATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG982850.14268955607396855Illumina Small RNA Adapter 2 (100% over 21bp)
ATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGA957520.13901216231565994RNA PCR Primer, Index 1 (100% over 22bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCTGGAATTCTCGGGTGCCAA898800.1304872289762252No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTGC874600.12697388792012299No Hit
TTCCATGGTGTAATGGTTAGCACTCTGGACTTGGAATTCTCGGGTGCCAA863750.12539869162017636No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAAT854470.12405142695072889No Hit
AGCAGAGTAGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA844900.12266206026036122No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGTGGAATTCTCGGGTGCCAAGG844200.12256043469262273Illumina Small RNA Adapter 2 (100% over 21bp)
TTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCCAA831490.12071520474125667No Hit
CGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAACT829830.12047420696633396RNA PCR Primer, Index 1 (100% over 25bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC804780.11683746343512072No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA795140.1154379341879792No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA782780.11364351702048237RNA PCR Primer, Index 1 (100% over 22bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTGCCA745740.10826607269329126No Hit
TCCCATGGTGTAATGGTTAGCACTCTGGACTTGGAATTCTCGGGTGCCAA745160.10818186865145078No Hit
TCCATGGTGTAATGGTTAGCACTCTGGACTTGGAATTCTCGGGTGCCAAG730940.10611741783253453No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGTGGAATTCTCGGGTGCCAA701830.10189124600843806No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)