FastQCFastQC Report
Tue 18 Jul 2023
SRR10854599.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10854599.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43635252
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length1-60
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCGGGTTTCGCCCCCGCGGCGTCGGGC3398890.7789321349628049No Hit
ACCTCTAGCATTACAAGTATTAGAGGC2849860.6531095546325709No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC2416870.5538801517635328No Hit
AAAAGCATCTGGCCTACACCCAGAAGAT1991030.45628933230407376No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC1890330.43321166106706566No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT1842900.4223420091626834No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTC1783040.4086237430231869No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGT1754110.4019937824582748No Hit
GGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCC1560410.35760306827149757No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCG1546050.3543121511020493No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC1525880.3496897416795026No Hit
AGGTCGCTGGTTCGTTTCCGGCTCGAAGGAC1499490.34364187927687456No Hit
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAC1459940.3345781067106018No Hit
AAAAGCATCTGGCCTACACCCAGAAGATT1447030.33161948967316607No Hit
AGAGTGGCGCAGCGGAAGCGTGCTGGGC1405780.3221661238486717No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT1240570.28430453432467856No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC1224250.28056443904575135No Hit
GGTGCCGTAACTTCGGGAGAAGGCACGC1218360.279214612992266No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC1191230.27299716293605913No Hit
AAGTTCAATTTTAAACTTGCTAAAAAAAC1169570.2680332864813065No Hit
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGT1119650.256592995039882No Hit
TGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGC1094810.2509003500197501No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGAT1062760.243555371239749No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGATT909750.20848968627475783No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCC905200.20744695137775304No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGC864960.19822504978314323No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC835650.1915080036663934No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC826560.1894248255974321No Hit
CCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGT820980.18814604302044594No Hit
CCCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGT818990.18768998973582185No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAC812770.1862645367557405No Hit
AGAGCCACCTTACAAATAAGCGCTCTC802240.18385135027981506No Hit
TAACCTAGACTTACAAGTCAAAGTAAAAT721090.1652540015123552No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGC704780.16151619795847633No Hit
CCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGT704530.16145890483226727No Hit
GAGAGAGAGACGGGGAGGGCGGCGCCGCCGC662440.15181303410371044No Hit
TCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGT643920.14756875931414354No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG642480.14723875090717936No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCC622310.14261634148463265No Hit
CCCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTC617000.14139943548395228No Hit
CCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTC605740.13881895307949638No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCC601160.13776934300734645No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGT591030.13544782553335546No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC588580.13488635289650672No Hit
AGAGTTGCGCAGCGGAAGCGTGCTGGGC586250.1343523809602383No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC565510.12959934320993494No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCG555060.12720449053439636No Hit
GGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCT550800.12622821566379405No Hit
GCGCCGGGTTTCGCCCCCGCGGCGTCGGG536880.12303813439647375No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAC536780.12301521714599013No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC529910.12144080203776524No Hit
CCCCGGGGAGCCCGGCGGGTGCCGGCGCGGGTCCCCTC503970.11549606726231351No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCC490870.11249390744895893No Hit
TGCGCCGGGTTTCGCCCCCGCGGCGTCGGGC478520.10966362701423152No Hit
TCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTC473760.10857276589121108No Hit
CCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTC471250.10799754290407215No Hit
ACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGC468370.10733752609014381No Hit
TTTCCATAGTGTAGCGGTTATCACGTCTGCT467430.10712210393559778No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAC467310.1070946032350174No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC458680.10511684451828077No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT451190.10340034245705744No Hit
TGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCT447110.10246531863732562No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCC446830.10240115033597148No Hit
GGTGCCGTAACTTCGGGAGAAGGCACGCT446440.10231177305908534No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAC441540.10118882778538785No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)