FastQCFastQC Report
Tue 18 Jul 2023
SRR10868219.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10868219.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12171975
Total Bases620.7 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG633460.5204249926573132TruSeq Adapter, Index 1 (100% over 49bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC354330.29110312829265583No Hit
CGCGCGGCGCCTCGCCTCGGCCGGCGCCTAGCAGCCGACTTAGAACTGGT289630.23794823765247627No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA191850.1576161633588633No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGG191790.1575668697972186No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC184500.15157770205739002No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC182780.15016461995690922No Hit
AGGGTCGGGCCTGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGG174770.14358392947734447No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC145030.11915075408879824No Hit
GTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGTACTTGGATGG126230.10370543810679861No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCC125110.10278529162276459No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT124670.10242380550403694No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)