FastQCFastQC Report
Tue 18 Jul 2023
SRR10868220.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10868220.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14449642
Total Bases736.9 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC950530.6578225259836887No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC359990.2491342000030174No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC350410.24250427796065813No Hit
CGCGCGGCGCCTCGCCTCGGCCGGCGCCTAGCAGCCGACTTAGAACTGGT309150.21394993730640524No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT285280.1974304968939715No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCC284050.1965792647319567No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAAC257270.1780459335947562No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC247930.17158210563279006No Hit
AAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGATTT212400.14699326114792324No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG190220.1316433998849245TruSeq Adapter, Index 2 (100% over 49bp)
TCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAG181380.12552560125711074No Hit
AAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCA178500.12353247229239313No Hit
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC170210.11779530593214696Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGTACTTGGATGG159500.11038335759460337No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA158090.10940755487229373No Hit
ATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAG154200.10671544665258835No Hit
AACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTC148280.10261845933622438No Hit
ATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCC147010.10173954482747738No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG145170.1004661568777967No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)