FastQCFastQC Report
Tue 18 Jul 2023
SRR10868221.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10868221.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15533836
Total Bases792.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGCGGCGCCTCGCCTCGGCCGGCGCCTAGCAGCCGACTTAGAACTGGT1363490.8777548572033333No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGG661230.42567077443073303No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC540630.34803380182461047No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC523370.336922573406852No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG373340.24033986196326523No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG339450.21852297140255633No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT336210.21643720198925753No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC331430.21336004834865002No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCC313390.20174669025731956No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC260820.16790443777055455No Hit
AAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGATTT246880.15893047924543557No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAAC235090.15134059610259823No Hit
AGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACACGTCTGAACTC215360.13863929038519526Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCG203730.13115240820103935No Hit
AGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGT197540.12716755861205178No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATT182180.11727946657863518No Hit
ATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAG168440.10843425925186798No Hit
AAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGATTTCTGCC164100.10564035824763439No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG156820.10095381462762966TruSeq Adapter, Index 3 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)