FastQCFastQC Report
Tue 18 Jul 2023
SRR10868223.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10868223.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16281485
Total Bases830.3 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC1077010.6614937151003117No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC372010.2284865293307091No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC327320.2010381731150445No Hit
CGCGCGGCGCCTCGCCTCGGCCGGCGCCTAGCAGCCGACTTAGAACTGGT287360.17649495730886955No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC276480.16981252017245355No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCC246440.15136211469654026No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG222350.13656616702960447TruSeq Adapter, Index 7 (100% over 49bp)
AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT219460.134791144665244No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAAC194810.11965124802805148No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA185450.11390238666804656No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGG173930.10682686499419432No Hit
AAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGATTT171530.10535279797880845No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG169370.10402613766496115No Hit
TCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAG165990.10195015995162604No Hit
GTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGTACTTGGATGG165840.10185803076316442No Hit
ACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCG162890.10004615672341928No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)