FastQCFastQC Report
Thu 24 Aug 2023
SRR10868297_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10868297_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6126311
Total Bases312.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGG160800.26247443200320714No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATG147400.24060156266960653TruSeq Adapter, Index 7 (97% over 36bp)
CGCGCGGCGCCTCGCCTCGGCCGGCGCCTAGCAGCCGACTTAGAACTGGT69180.11292276869391711No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC65850.10748719743414921No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC65010.10611606234159512No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC61610.10056622982411438No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)