FastQCFastQC Report
Thu 24 Aug 2023
SRR10868305_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10868305_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7826332
Total Bases399.1 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATG277170.3541505778185745TruSeq Adapter, Index 9 (97% over 36bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGCCGTC129710.16573536619708953RNA PCR Primer, Index 35 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGA81930.10468505552792802No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)