FastQCFastQC Report
Thu 24 Aug 2023
SRR10868312_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10868312_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14570269
Total Bases743 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA333020.22856132580668206No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC326000.22374329533655143No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC295240.2026318113962069No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGG273050.18740216807253182No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG176470.12111650100626145Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGCGGCGCCTCGCCTCGGCCGGCGCCTAGCAGCCGACTTAGAACTGGT164920.11318939959173026No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)