FastQCFastQC Report
Thu 24 Aug 2023
SRR10883784_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10883784_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29296924
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length62-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAAC4775411.6300038870974987RNA PCR Primer, Index 1 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG3862551.3184148615738636No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAACT3527631.2040956927764839RNA PCR Primer, Index 1 (100% over 25bp)
ACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAACTC2999161.023711567808279RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC2802380.9565441068147632No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA2232490.7620219788261731No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC1999230.6824026986587397RNA PCR Primer, Index 1 (100% over 24bp)
ACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAAC1550870.5293627412898364RNA PCR Primer, Index 1 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC1544800.527290851421808No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG1335420.4558225976215114Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA1299740.4436438446575483No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCAA1295510.44220000707241486No Hit
GTACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTC1150180.39259411670658667RNA PCR Primer, Index 1 (100% over 26bp)
TCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAACT1125270.3840915175941338RNA PCR Primer, Index 1 (100% over 25bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA1018600.3476815518243485RNA PCR Primer, Index 3 (100% over 36bp)
ACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA928890.31706058970559503RNA PCR Primer, Index 1 (100% over 28bp)
ACGGAGTCAAGACCCGCAACGCCTGGAATTCTCGGGTGCCAAGGAACTCC902740.3081347379677129RNA PCR Primer, Index 1 (100% over 27bp)
AAATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCCA896810.30611063468642646RNA PCR Primer, Index 1 (100% over 28bp)
ACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAACTC833950.28465445723926514RNA PCR Primer, Index 1 (100% over 26bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCCAAGGAA758440.258880420347201RNA PCR Primer, Index 1 (100% over 23bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCC681170.23250563779323727No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGATGGAATTCTCGGGTGCCAAGGA652320.22265818759675932RNA PCR Primer, Index 1 (100% over 22bp)
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA648360.22130650985748537RNA PCR Primer, Index 1 (100% over 22bp)
ACGGAGTCAAGACCCGCAACGCCCTGGAATTCTCGGGTGCCAAGGAACTC647280.22093787047404706RNA PCR Primer, Index 1 (100% over 26bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA636580.2172856099159079No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGCC632880.21602267869486913No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG631980.21571547920867049Illumina Small RNA Adapter 2 (100% over 21bp)
CTGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG589110.20108254368274292No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA564480.19267551774377406RNA PCR Primer, Index 1 (100% over 23bp)
ACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCA551220.18814944531378108RNA PCR Primer, Index 1 (100% over 28bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC539570.18417291863132118No Hit
ACACGGAGTCAAGACCCGCAACGCCCTGGAATTCTCGGGTGCCAAGGAAC530750.18116236366657468RNA PCR Primer, Index 1 (100% over 24bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCTGGAATTCTCGGGTGC523790.17878668763997202No Hit
ACCCCTCCCACGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT496820.16958094303688676RNA PCR Primer, Index 3 (100% over 34bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA488810.16684686760971904No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAAC486990.16622564198207293RNA PCR Primer, Index 1 (100% over 24bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC443440.15136060017768418No Hit
CAGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG429190.1464966083128727No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCCA411760.14054717826349278No Hit
CAGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA404970.1382295288065054RNA PCR Primer, Index 1 (100% over 22bp)
AATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCCAG398360.1359733192467578RNA PCR Primer, Index 1 (100% over 29bp)
AGGATAGCTGGCGCTCTCGCAGACCCGTGGAATTCTCGGGTGCCAAGGAA394000.1344851084025067RNA PCR Primer, Index 1 (100% over 23bp)
ACACGGAGTCAAGACCCGCAACGCCTGGAATTCTCGGGTGCCAAGGAACT390160.1331743905947259RNA PCR Primer, Index 1 (100% over 25bp)
ACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT368590.12581184290883235RNA PCR Primer, Index 3 (100% over 35bp)
GTCGGGCCCGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA366080.12495509767510063RNA PCR Primer, Index 3 (100% over 36bp)
CCGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG353840.12077718466279941No Hit
AGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT349820.11940502695777892RNA PCR Primer, Index 1 (100% over 30bp)
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT347500.11861313494891136RNA PCR Primer, Index 1 (100% over 25bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG346380.1182308422549753No Hit
GTGGGGGGGCCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT340660.11627841885380186RNA PCR Primer, Index 3 (100% over 34bp)
ACCCCTCCCACGGCGCGTGGGGGGGCCGGGCTGGAATTCTCGGGTGCCAA335830.11462978161120259No Hit
CACCCCTCCCACGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC335580.11454444842059186RNA PCR Primer, Index 1 (100% over 33bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGTGGAATTCTCGGGTGCCAA332070.11334637042441724No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG328800.11223021229122894No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA326350.1113939470232438No Hit
TAAATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCC323010.11025389559668448RNA PCR Primer, Index 1 (100% over 27bp)
TTGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG322550.11009688252596075No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA319340.109001204358519No Hit
ACACGGAGTCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA319020.10889197787453728RNA PCR Primer, Index 3 (100% over 36bp)
TCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAACTC315600.10772461982698253RNA PCR Primer, Index 1 (100% over 26bp)
AAATGGATTTTTGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC314970.10750958018664349RNA PCR Primer, Index 1 (100% over 33bp)
CTGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA312750.10675182145402022RNA PCR Primer, Index 1 (100% over 22bp)
TAGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA308740.10538307707662416RNA PCR Primer, Index 1 (100% over 22bp)
CTCGGTTGGCCTCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAACT300620.10261145504558772RNA PCR Primer, Index 1 (100% over 25bp)
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC297700.10161476337925443RNA PCR Primer, Index 1 (100% over 24bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAG297560.10156697679251242No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAA297460.10153284351626812No Hit
AATGGATTTTTGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT295500.10086383130188002RNA PCR Primer, Index 3 (100% over 34bp)
ACACGGAGTCGACCCGCAACGCTCTGGAATTCTCGGGTGCCAAGGAACTC294130.10039620541733323RNA PCR Primer, Index 1 (100% over 26bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGG294020.10035865881346451Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)