FastQCFastQC Report
Thu 24 Aug 2023
SRR10883785_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10883785_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28567855
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAAC4656851.63010138493072RNA PCR Primer, Index 1 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG3812771.3346364296514388No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAACT3465661.2131327325765269RNA PCR Primer, Index 1 (100% over 25bp)
ACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAACTC2972701.0405751499368783RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC2784420.9746689067135071No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA2140350.7492162082172428No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC2010730.7038435332299188RNA PCR Primer, Index 1 (100% over 24bp)
ACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAAC1536270.537761760552201RNA PCR Primer, Index 1 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC1506600.5273759615483906No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG1321350.4625303509836493Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA1271500.4450806684646082No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCAA1262280.4418532647970945No Hit
GTACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTC1107170.38755797381357476RNA PCR Primer, Index 1 (100% over 26bp)
TCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAACT1097240.38408203906103555RNA PCR Primer, Index 1 (100% over 25bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA1012320.35435632111686366RNA PCR Primer, Index 3 (100% over 36bp)
ACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA917800.3212701828681222RNA PCR Primer, Index 1 (100% over 28bp)
AAATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCCA888950.31117141976532714RNA PCR Primer, Index 1 (100% over 28bp)
ACGGAGTCAAGACCCGCAACGCCTGGAATTCTCGGGTGCCAAGGAACTCC886920.31046083088842336RNA PCR Primer, Index 1 (100% over 27bp)
ACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAACTC821300.2874909579315633RNA PCR Primer, Index 1 (100% over 26bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCCAAGGAA744080.26046057710668163RNA PCR Primer, Index 1 (100% over 23bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCC685330.23989550493027914No Hit
ACGGAGTCAAGACCCGCAACGCCCTGGAATTCTCGGGTGCCAAGGAACTC646010.22613178343281284RNA PCR Primer, Index 1 (100% over 26bp)
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA638260.22341894412443636RNA PCR Primer, Index 1 (100% over 22bp)
AGGATAGCTGGCGCTCTCGCAGACCCGATGGAATTCTCGGGTGCCAAGGA637370.22310740515870026RNA PCR Primer, Index 1 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG622480.21789525324880007Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA621900.21769222785539902No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGCC618180.2163900649873783No Hit
CTGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG581600.20358546345184123No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA558660.19555545909904684RNA PCR Primer, Index 1 (100% over 23bp)
ACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCA536750.18788599984143017RNA PCR Primer, Index 1 (100% over 28bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC528550.18501564083127697No Hit
ACACGGAGTCAAGACCCGCAACGCCCTGGAATTCTCGGGTGCCAAGGAAC523510.18325142017137794RNA PCR Primer, Index 1 (100% over 24bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCTGGAATTCTCGGGTGC510040.17853633043152872No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA496860.17392275338838006No Hit
ACCCCTCCCACGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT488350.1709438808058918RNA PCR Primer, Index 3 (100% over 34bp)
TCTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAAC473900.1658857481599511RNA PCR Primer, Index 1 (100% over 24bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC424590.14862508928304208No Hit
CAGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG421610.1475819588134986No Hit
CAGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA401110.1404060612881156RNA PCR Primer, Index 1 (100% over 22bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCCA396110.1386558423794856No Hit
AATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCCAG391980.13721016156095722RNA PCR Primer, Index 1 (100% over 29bp)
AGGATAGCTGGCGCTCTCGCAGACCCGTGGAATTCTCGGGTGCCAAGGAA385520.13494887873100728RNA PCR Primer, Index 1 (100% over 23bp)
ACACGGAGTCAAGACCCGCAACGCCTGGAATTCTCGGGTGCCAAGGAACT383450.13422428810283446RNA PCR Primer, Index 1 (100% over 25bp)
GTCGGGCCCGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA359830.12595625397846635RNA PCR Primer, Index 3 (100% over 36bp)
CCGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG352460.12337643130714575No Hit
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT343790.12034155171958133RNA PCR Primer, Index 1 (100% over 25bp)
AGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT342650.11994250180841369RNA PCR Primer, Index 1 (100% over 30bp)
ACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT341280.11946294182744908RNA PCR Primer, Index 3 (100% over 35bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG338700.11855982887059599No Hit
CACCCCTCCCACGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC332420.1163615539213567RNA PCR Primer, Index 1 (100% over 33bp)
GTGGGGGGGCCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT331800.11614452677668659RNA PCR Primer, Index 3 (100% over 34bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA329800.1154444392132346No Hit
ACCCCTCCCACGGCGCGTGGGGGGGCCGGGCTGGAATTCTCGGGTGCCAA327030.11447481793785358No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGTGGAATTCTCGGGTGCCAA325740.11402326145942704No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG322030.11272459902922358No Hit
TTGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG321280.11246206619292909No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA320230.11209452022211679No Hit
TAAATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCC318750.11157645542516231RNA PCR Primer, Index 1 (100% over 27bp)
TCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAACTC315060.11028479387059337RNA PCR Primer, Index 1 (100% over 26bp)
CTGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA313270.10965821550130382RNA PCR Primer, Index 1 (100% over 22bp)
AAATGGATTTTTGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC309920.10848556883252174RNA PCR Primer, Index 1 (100% over 33bp)
TAGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA308080.10784148827414589RNA PCR Primer, Index 1 (100% over 22bp)
ACACGGAGTCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA306620.10733042435282594RNA PCR Primer, Index 3 (100% over 36bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAA299300.1047681038705916No Hit
CTCGGTTGGCCTCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAACT297750.1042255360089163RNA PCR Primer, Index 1 (100% over 25bp)
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC293690.10280435825510877RNA PCR Primer, Index 1 (100% over 24bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGG292950.10254532585663152Illumina Small RNA Adapter 2 (100% over 21bp)
AATGGATTTTTGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT292770.10248231797592083RNA PCR Primer, Index 3 (100% over 34bp)
ACACGGAGTCGACCCGCAACGCTCTGGAATTCTCGGGTGCCAAGGAACTC291360.10198875624368717RNA PCR Primer, Index 1 (100% over 26bp)
TTGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA288370.10094212533632643RNA PCR Primer, Index 1 (100% over 22bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAG287720.10071459687820454No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAACTC285740.10002151019038706RNA PCR Primer, Index 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)