FastQCFastQC Report
Mon 24 Jul 2023
SRR10883786_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10883786_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29001532
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length64-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAAC4864631.6773700092808892RNA PCR Primer, Index 1 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG3889781.3412325941953689No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAACT3585281.236238140798907RNA PCR Primer, Index 1 (100% over 25bp)
ACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAACTC2939831.013680932441776RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC2761580.9522186621037814No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA2280430.786313633362541No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC2086730.7195240582463023RNA PCR Primer, Index 1 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC1544890.5326925487936293No Hit
ACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAAC1470270.5069628735475078RNA PCR Primer, Index 1 (100% over 24bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG1356940.46788562755926133Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA1333080.4596584759729245No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCAA1308540.4511968540144707No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAACT1150560.3967238696217841RNA PCR Primer, Index 1 (100% over 25bp)
GTACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTC1137040.39206204692910707RNA PCR Primer, Index 1 (100% over 26bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA1047210.3610878211537239RNA PCR Primer, Index 3 (100% over 36bp)
ACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA898350.30975949822236976RNA PCR Primer, Index 1 (100% over 28bp)
ACGGAGTCAAGACCCGCAACGCCTGGAATTCTCGGGTGCCAAGGAACTCC882480.30428737350840634RNA PCR Primer, Index 1 (100% over 27bp)
AAATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCCA864440.2980670124598935RNA PCR Primer, Index 1 (100% over 28bp)
ACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAACTC797610.2750234022119935RNA PCR Primer, Index 1 (100% over 26bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCCAAGGAA779550.2687961449760654RNA PCR Primer, Index 1 (100% over 23bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCC713780.24611803266117116No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGATGGAATTCTCGGGTGCCAAGGA665090.22932926439886003RNA PCR Primer, Index 1 (100% over 22bp)
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA657030.22655010087053334RNA PCR Primer, Index 1 (100% over 22bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGCC644990.22239859604658127No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG636870.21959874395600898Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA631060.21759540151189255No Hit
ACGGAGTCAAGACCCGCAACGCCCTGGAATTCTCGGGTGCCAAGGAACTC624150.21521276875994RNA PCR Primer, Index 1 (100% over 26bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA587340.2025203358222593RNA PCR Primer, Index 1 (100% over 23bp)
CTGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG578210.19937222626721923No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC549430.1894486125767425No Hit
ACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCA543380.18736251588364367RNA PCR Primer, Index 1 (100% over 28bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCTGGAATTCTCGGGTGC530910.18306274303026476No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAAC496850.17131853586217446RNA PCR Primer, Index 1 (100% over 24bp)
ACACGGAGTCAAGACCCGCAACGCCCTGGAATTCTCGGGTGCCAAGGAAC493780.1702599710939408RNA PCR Primer, Index 1 (100% over 24bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA484060.1669084240101523No Hit
ACCCCTCCCACGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT447860.1544263247886353RNA PCR Primer, Index 3 (100% over 34bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC444560.15328845386512685No Hit
CAGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG417690.14402342607280197No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCCA413980.14274418330728184No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGTGGAATTCTCGGGTGCCAAGGAA395320.13631004044889766RNA PCR Primer, Index 1 (100% over 23bp)
CAGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA390100.13451013553352975RNA PCR Primer, Index 1 (100% over 22bp)
AATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCCAG386980.1334343302967581RNA PCR Primer, Index 1 (100% over 29bp)
GTCGGGCCCGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA372800.12854493341937936RNA PCR Primer, Index 3 (100% over 36bp)
ACACGGAGTCAAGACCCGCAACGCCTGGAATTCTCGGGTGCCAAGGAACT365940.12617954113596483RNA PCR Primer, Index 1 (100% over 25bp)
ACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT357190.12316245914181362RNA PCR Primer, Index 3 (100% over 35bp)
CCGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG354030.12207286153021159No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG347710.1198936663070075No Hit
GTGGGGGGGCCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT346380.11943506984389651RNA PCR Primer, Index 3 (100% over 34bp)
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT345850.11925232087739365RNA PCR Primer, Index 1 (100% over 25bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGTGGAATTCTCGGGTGCCAA345330.11907302000459838No Hit
AGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT341960.11791101242513671RNA PCR Primer, Index 1 (100% over 30bp)
ACCCCTCCCACGGCGCGTGGGGGGGCCGGGCTGGAATTCTCGGGTGCCAA341120.1176213725536982No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA339970.11722484177732403No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG335910.11582491573203788No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA333390.11495599611772234No Hit
TTGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG327270.1128457627686703No Hit
TCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAACTC322890.11133549772474088RNA PCR Primer, Index 1 (100% over 26bp)
CTCGGTTGGCCTCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAACT314780.10853909372787617RNA PCR Primer, Index 1 (100% over 25bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAA310380.10702193249653155No Hit
TAAATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCC310320.10700124393428596RNA PCR Primer, Index 1 (100% over 27bp)
CTGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA307780.1061254281325552RNA PCR Primer, Index 1 (100% over 22bp)
TAGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA302590.1043358674983101RNA PCR Primer, Index 1 (100% over 22bp)
AAATGGATTTTTGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC300840.10373245109947984RNA PCR Primer, Index 1 (100% over 33bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGG300470.10360487163229859Illumina Small RNA Adapter 2 (100% over 21bp)
ACACGGAGTCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA300300.10354625403926937RNA PCR Primer, Index 3 (100% over 36bp)
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC298960.1030842094824508RNA PCR Primer, Index 1 (100% over 24bp)
CACCCCTCCCACGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC293760.10129120075449806RNA PCR Primer, Index 1 (100% over 33bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAG293480.10119465413068524No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAA293260.101118796069118RNA PCR Primer, Index 1 (100% over 23bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGGAATTCTCGGGTG291480.10050503538916497No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)