FastQCFastQC Report
Thu 24 Aug 2023
SRR10883787_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10883787_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28950624
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAAC4725371.632216977430262RNA PCR Primer, Index 1 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG3843451.3275879649433462No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAACT3513321.2135558805226443RNA PCR Primer, Index 1 (100% over 25bp)
ACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAACTC2981681.0299190787735697RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC2775210.9586010995825168No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA2178810.7525951772231231No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC2016900.696668921540344RNA PCR Primer, Index 1 (100% over 24bp)
ACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAAC1541170.5323443114732174RNA PCR Primer, Index 1 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC1515650.523529302857168No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG1331550.459938272833083Illumina Small RNA Adapter 2 (100% over 21bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCAA1284020.4435206647013895No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA1277130.44114075054133545No Hit
GTACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTC1128430.38977743623073546RNA PCR Primer, Index 1 (100% over 26bp)
TCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAACT1119970.38685521942463136RNA PCR Primer, Index 1 (100% over 25bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA1030200.3558472522043048RNA PCR Primer, Index 3 (100% over 36bp)
ACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA914430.31585847683283097RNA PCR Primer, Index 1 (100% over 28bp)
ACGGAGTCAAGACCCGCAACGCCTGGAATTCTCGGGTGCCAAGGAACTCC904540.3124423155784138RNA PCR Primer, Index 1 (100% over 27bp)
AAATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCCA898930.3105045335119547RNA PCR Primer, Index 1 (100% over 28bp)
ACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAACTC819310.28300253562755673RNA PCR Primer, Index 1 (100% over 26bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCCAAGGAA756050.2611515385644192RNA PCR Primer, Index 1 (100% over 23bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCC695560.24025734298507695No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGATGGAATTCTCGGGTGCCAAGGA652010.22521448933190527RNA PCR Primer, Index 1 (100% over 22bp)
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA644850.22274131293335853RNA PCR Primer, Index 1 (100% over 22bp)
ACGGAGTCAAGACCCGCAACGCCCTGGAATTCTCGGGTGCCAAGGAACTC643460.2222611851129703RNA PCR Primer, Index 1 (100% over 26bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGCC632320.2184132542359018No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG627770.21684161280945102Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA622390.21498327635355977No Hit
CTGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG588400.20324259677442533No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA560570.19362967789571653RNA PCR Primer, Index 1 (100% over 23bp)
ACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCA547120.1889838367559884RNA PCR Primer, Index 1 (100% over 28bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC536710.1853880593385483No Hit
ACACGGAGTCAAGACCCGCAACGCCCTGGAATTCTCGGGTGCCAAGGAAC527420.18217914750300374RNA PCR Primer, Index 1 (100% over 24bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCTGGAATTCTCGGGTGC513090.1772293405489291No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA491470.16976145315555202No Hit
ACCCCTCCCACGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT487160.1682727114966503RNA PCR Primer, Index 3 (100% over 34bp)
TCTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAAC485520.16770622975173177RNA PCR Primer, Index 1 (100% over 24bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC429840.14847348368035176No Hit
CAGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG429620.14839749222676513No Hit
CAGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA407250.14067054305979726RNA PCR Primer, Index 1 (100% over 22bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCCA401760.1387742108771127No Hit
AATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCCAG393350.1358692648559147RNA PCR Primer, Index 1 (100% over 29bp)
AGGATAGCTGGCGCTCTCGCAGACCCGTGGAATTCTCGGGTGCCAAGGAA391350.13517843345967256RNA PCR Primer, Index 1 (100% over 23bp)
ACACGGAGTCAAGACCCGCAACGCCTGGAATTCTCGGGTGCCAAGGAACT388550.13421126950493364RNA PCR Primer, Index 1 (100% over 25bp)
GTCGGGCCCGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA368860.12741003440893017RNA PCR Primer, Index 3 (100% over 36bp)
CCGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG356170.12302670919977407No Hit
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT347480.12002504678310215RNA PCR Primer, Index 1 (100% over 25bp)
AGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT345250.11925476977629221RNA PCR Primer, Index 1 (100% over 30bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG341280.11788346945475164No Hit
ACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT341050.11780402384418381RNA PCR Primer, Index 3 (100% over 35bp)
GTGGGGGGGCCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT332500.11485071962524884RNA PCR Primer, Index 3 (100% over 34bp)
ACCCCTCCCACGGCGCGTGGGGGGGCCGGGCTGGAATTCTCGGGTGCCAA330940.11431187113618001No Hit
CACCCCTCCCACGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC329250.11372811860635543RNA PCR Primer, Index 1 (100% over 33bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGTGGAATTCTCGGGTGCCAA328130.11334125302445985No Hit
TTGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG327880.1132548990999296No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA327260.11304074136709454No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA324710.11215993133688587No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG324200.11198376933084413No Hit
TAAATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCC319940.11051229845684846RNA PCR Primer, Index 1 (100% over 27bp)
TCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAACTC317310.1096038551707901RNA PCR Primer, Index 1 (100% over 26bp)
CTGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA314290.10856069976246452RNA PCR Primer, Index 1 (100% over 22bp)
AAATGGATTTTTGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC310230.10715831202809308RNA PCR Primer, Index 1 (100% over 33bp)
TAGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA308360.10651238467260671RNA PCR Primer, Index 1 (100% over 22bp)
ACACGGAGTCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA308310.10649511388770067RNA PCR Primer, Index 3 (100% over 36bp)
CTCGGTTGGCCTCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAACT304320.10511690525219768RNA PCR Primer, Index 1 (100% over 25bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAA303310.10476803539709542No Hit
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC298730.10318603149970101RNA PCR Primer, Index 1 (100% over 24bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGG296740.10249865426044012Illumina Small RNA Adapter 2 (100% over 21bp)
AATGGATTTTTGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT295400.10203579722495792RNA PCR Primer, Index 3 (100% over 34bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAG294910.1018665435328786No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)