FastQCFastQC Report
Thu 24 Aug 2023
SRR10883788_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10883788_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29965457
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG4937641.6477773057157115No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAAC2798590.9339387014855137RNA PCR Primer, Index 1 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA2315460.7727097237328968No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAACT1909870.6373572076674819RNA PCR Primer, Index 1 (100% over 25bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC1899200.6337964410153998RNA PCR Primer, Index 4 (100% over 50bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGCC1583110.528311648976353No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCTGGAATTCTCGGGTGC1410810.47081210875575835No Hit
ACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAACTC1151940.38442263703837387RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC1091290.3641826653936898No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC1086850.36270095930791246RNA PCR Primer, Index 1 (100% over 24bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCCA1074840.35869301108940205No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC1060330.3538507689036747No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAACT1057850.3530231492881954RNA PCR Primer, Index 1 (100% over 25bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG1054170.351795068568452Illumina Small RNA Adapter 2 (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA1044730.34864477454824067No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG1000160.33377098170069625Illumina Small RNA Adapter 2 (100% over 21bp)
CTGGTCCGATGGTAGTGGGTTATCTGGAATTCTCGGGTGCCAAGGAACTC891670.29756596069934793RNA PCR Primer, Index 1 (100% over 26bp)
ACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG801050.2673244729756666RNA PCR Primer, Index 4 (100% over 35bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC789010.2633065132295496No Hit
GCTGGTCCGATGGTAGTGGGTTATCTGGAATTCTCGGGTGCCAAGGAACT782730.26121076678390054RNA PCR Primer, Index 1 (100% over 25bp)
CTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGGA770310.25706599435476657RNA PCR Primer, Index 1 (100% over 22bp)
GTACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTC747830.24956402300155145RNA PCR Primer, Index 1 (100% over 26bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAG737420.2460900229220599No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA701410.2340728526182664No Hit
ACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA658970.21990987823079086RNA PCR Primer, Index 1 (100% over 28bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG645900.21554818937018047RNA PCR Primer, Index 4 (100% over 45bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA638870.2132021547343663No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGG637980.21290514608203703Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG630650.21045899617015687No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCAA613700.2048024830724257No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA609750.20348429860422285No Hit
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA606460.2023863677433653RNA PCR Primer, Index 4 (100% over 36bp)
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA598500.19972997575174642RNA PCR Primer, Index 1 (100% over 22bp)
ACGGAGTCAAGACCCGCAACGCCTGGAATTCTCGGGTGCCAAGGAACTCC597530.19940626969246622RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA576380.19234814272981052No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC547260.18263028659966707No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTTGGAATTCTCGGGTG459770.15343333492294145No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAAG451060.15052665474115745No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGTGGAATTCTCGGGTGCCAA446020.148844718103248No Hit
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG442950.14782020511150556RNA PCR Primer, Index 4 (100% over 45bp)
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT414830.1384360665682489RNA PCR Primer, Index 1 (100% over 25bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCCAAGGAA413920.13813238356418192RNA PCR Primer, Index 1 (100% over 23bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGTGGAATTCTCGGGTG409530.1366673633577489No Hit
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC402370.13427794543563945RNA PCR Primer, Index 1 (100% over 24bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGG389870.13010647559955452Illumina Small RNA Adapter 2 (100% over 21bp)
ACGGAGTCAAGACCCGCAACGCCCTGGAATTCTCGGGTGCCAAGGAACTC384280.12824099428885735RNA PCR Primer, Index 1 (100% over 26bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGCCA381840.12742672337685354No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA378960.1264656167266196RNA PCR Primer, Index 1 (100% over 23bp)
AATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCCAG358370.11959437161262049RNA PCR Primer, Index 1 (100% over 29bp)
ACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCA357330.11924730532225823RNA PCR Primer, Index 1 (100% over 28bp)
AGTTGGTCCGAGTGTTGTGGGTTTGGAATTCTCGGGTGCCAAGGAACTCC356240.11888355315255161RNA PCR Primer, Index 1 (100% over 27bp)
ACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAACTC353930.11811266552684313RNA PCR Primer, Index 1 (100% over 26bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG346740.11571323607712707No Hit
AAATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCCA339170.11318699394439403RNA PCR Primer, Index 1 (100% over 28bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA337910.11276650978491669No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG330370.11025027917979024Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGTGGAATTCTCGGGTGCCA321820.10739699381190818No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAAC307730.10269491301267322RNA PCR Primer, Index 1 (100% over 24bp)
AGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT300720.10035555272859678RNA PCR Primer, Index 1 (100% over 30bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG300470.1002721233318751No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)