FastQCFastQC Report
Thu 24 Aug 2023
SRR10883789_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10883789_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29349672
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG4894901.6677869517587793No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAAC2761980.9410599205333539RNA PCR Primer, Index 1 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA2258280.7694396039587768No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAACT1884530.6420957617516134RNA PCR Primer, Index 1 (100% over 25bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC1862250.6345045355191704RNA PCR Primer, Index 4 (100% over 50bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGCC1545160.5264658494309579No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCTGGAATTCTCGGGTGC1384370.47168159153533296No Hit
ACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAACTC1146540.3906483179777954RNA PCR Primer, Index 1 (100% over 26bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC1089250.3711285086933851RNA PCR Primer, Index 1 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC1085080.3697077091696289No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG1049470.3575746945315096Illumina Small RNA Adapter 2 (100% over 21bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCCA1047780.35699887889718157No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAACT1039300.3541095791462337RNA PCR Primer, Index 1 (100% over 25bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC1038060.35368708720151965No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA1019830.3474757741756024No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG997490.3398641047845441Illumina Small RNA Adapter 2 (100% over 21bp)
CTGGTCCGATGGTAGTGGGTTATCTGGAATTCTCGGGTGCCAAGGAACTC885300.30163880536722865RNA PCR Primer, Index 1 (100% over 26bp)
GCTGGTCCGATGGTAGTGGGTTATCTGGAATTCTCGGGTGCCAAGGAACT780500.2659314216526849RNA PCR Primer, Index 1 (100% over 25bp)
ACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG778500.26524998303217834RNA PCR Primer, Index 4 (100% over 35bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC771400.2628308759293801No Hit
CTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGGA770000.2623538688950255RNA PCR Primer, Index 1 (100% over 22bp)
GTACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTC727450.2478562622437484RNA PCR Primer, Index 1 (100% over 26bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAG723710.24658197202340115No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA686440.2338833633302614No Hit
ACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA661910.22552551864974843RNA PCR Primer, Index 1 (100% over 28bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG656110.22354934665027942RNA PCR Primer, Index 4 (100% over 45bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA652320.22225802046441948No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGG640660.21828523330686625Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG622550.21211480659817936No Hit
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA608450.20731066432360812RNA PCR Primer, Index 4 (100% over 36bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCAA603170.2055116663654708No Hit
ACGGAGTCAAGACCCGCAACGCCTGGAATTCTCGGGTGCCAAGGAACTCC593770.20230890484908998RNA PCR Primer, Index 1 (100% over 27bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA591710.2016070230699682No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA591030.20137533393899598RNA PCR Primer, Index 1 (100% over 22bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA566630.19306178276881594No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC533800.18187596781320076No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTTGGAATTCTCGGGTG449900.1532896176829506No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAAG447130.15234582519354903No Hit
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG435290.14831170856015016RNA PCR Primer, Index 4 (100% over 45bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGTGGAATTCTCGGGTGCCAA429840.1464547883192698No Hit
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT409080.1393814554384117RNA PCR Primer, Index 1 (100% over 25bp)
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC403670.13753816396994148RNA PCR Primer, Index 1 (100% over 24bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCCAAGGAA401950.13695212675630583RNA PCR Primer, Index 1 (100% over 23bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGTGGAATTCTCGGGTG397400.13540185389465342No Hit
ACGGAGTCAAGACCCGCAACGCCCTGGAATTCTCGGGTGCCAAGGAACTC387540.13204236149555607RNA PCR Primer, Index 1 (100% over 26bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGG383210.13056704688215937Illumina Small RNA Adapter 2 (100% over 21bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGCCA371960.12673395464180998No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA370810.1263421274350187RNA PCR Primer, Index 1 (100% over 23bp)
ACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCA355530.12113593637434858RNA PCR Primer, Index 1 (100% over 28bp)
AATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCCAG354080.12064189337448131RNA PCR Primer, Index 1 (100% over 29bp)
AGTTGGTCCGAGTGTTGTGGGTTTGGAATTCTCGGGTGCCAAGGAACTCC353810.12054989916071294RNA PCR Primer, Index 1 (100% over 27bp)
ACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAACTC351480.11975602316782279RNA PCR Primer, Index 1 (100% over 26bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA347670.1184578825957578No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG342230.11660436954797995No Hit
AAATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCCA341740.11643741708595584RNA PCR Primer, Index 1 (100% over 28bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG325930.1110506447908515Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGTGGAATTCTCGGGTGCCA319290.1087882685707697No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAAC306800.10453268438570625RNA PCR Primer, Index 1 (100% over 24bp)
AGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT295680.10074388565568977RNA PCR Primer, Index 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)