FastQCFastQC Report
Thu 24 Aug 2023
SRR10883790_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10883790_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30709667
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG5114391.6654006700886725No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAAC2888530.9405930712306323RNA PCR Primer, Index 1 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA2387350.7773936461114996No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAACT1982430.6455393996945652RNA PCR Primer, Index 1 (100% over 25bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC1847140.6014848679407693RNA PCR Primer, Index 4 (100% over 50bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGCC1627180.5298592133871071No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCTGGAATTCTCGGGTGC1449030.4718481642930221No Hit
ACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAACTC1181430.38470947926592625RNA PCR Primer, Index 1 (100% over 26bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC1148200.3738887823173075RNA PCR Primer, Index 1 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC1114570.3629378332236556No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG1102760.3590921386415554Illumina Small RNA Adapter 2 (100% over 21bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCCA1098580.35773100372596034No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAACT1092370.35570883917432256RNA PCR Primer, Index 1 (100% over 25bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC1084260.3530679769337779No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA1082010.3523353086179671No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG1046370.3407298424955243Illumina Small RNA Adapter 2 (100% over 21bp)
CTGGTCCGATGGTAGTGGGTTATCTGGAATTCTCGGGTGCCAAGGAACTC918920.29922825278437565RNA PCR Primer, Index 1 (100% over 26bp)
ACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG827020.2693028224630375RNA PCR Primer, Index 4 (100% over 35bp)
GCTGGTCCGATGGTAGTGGGTTATCTGGAATTCTCGGGTGCCAAGGAACT813730.2649751949443151RNA PCR Primer, Index 1 (100% over 25bp)
CTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGGA805820.26239945877628695RNA PCR Primer, Index 1 (100% over 22bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC800230.2605791850494504No Hit
GTACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTC769340.2505204631492748RNA PCR Primer, Index 1 (100% over 26bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAG767350.24987245872773547No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA724880.2360429372288537No Hit
ACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA678250.22085879342162842RNA PCR Primer, Index 1 (100% over 28bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGG672790.21908085164192762Illumina Small RNA Adapter 2 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA665140.21658977936817092No Hit
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG654110.2129980764688852RNA PCR Primer, Index 4 (100% over 45bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA634290.20654408268249866RNA PCR Primer, Index 4 (100% over 36bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG632270.20588630935008184No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCAA630560.20532948143006566No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA619190.20162706420750182No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA617470.20106697998385983RNA PCR Primer, Index 1 (100% over 22bp)
ACGGAGTCAAGACCCGCAACGCCTGGAATTCTCGGGTGCCAAGGAACTCC612580.19947464751083102RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA603340.1964658229605681No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC557960.18168871710657103No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTTGGAATTCTCGGGTG484100.15763765852622239No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAAG470180.153104883879073No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGTGGAATTCTCGGGTGCCAA463130.15080918982286587No Hit
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG446240.14530929299884626RNA PCR Primer, Index 4 (100% over 45bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCCAAGGAA426130.13876086640731078RNA PCR Primer, Index 1 (100% over 23bp)
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT426090.13874784119280748RNA PCR Primer, Index 1 (100% over 25bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGTGGAATTCTCGGGTG422230.13749090799323874No Hit
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC415330.13524405849141902RNA PCR Primer, Index 1 (100% over 24bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGG405760.13212777592150382Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA400070.130274939158409RNA PCR Primer, Index 1 (100% over 23bp)
ACGGAGTCAAGACCCGCAACGCCCTGGAATTCTCGGGTGCCAAGGAACTC394430.12843838391344328RNA PCR Primer, Index 1 (100% over 26bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGCCA393470.12812577876536402No Hit
ACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCA373880.12174667996237146RNA PCR Primer, Index 1 (100% over 28bp)
AATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCCAG367410.11963985151646223RNA PCR Primer, Index 1 (100% over 29bp)
AGTTGGTCCGAGTGTTGTGGGTTTGGAATTCTCGGGTGCCAAGGAACTCC367190.11956821283669405RNA PCR Primer, Index 1 (100% over 27bp)
ACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAACTC359220.11697293884691097RNA PCR Primer, Index 1 (100% over 26bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG357640.11645844287403051No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA356860.1162044511912161No Hit
AAATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCCA348150.11336821073312192RNA PCR Primer, Index 1 (100% over 28bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGTGGAATTCTCGGGTGCCA337630.10994257931875329No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG333330.10854236875964822Illumina Small RNA Adapter 2 (100% over 21bp)
CCTCGGTTGGCCTCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAAC323260.10526327100844174RNA PCR Primer, Index 1 (100% over 24bp)
AGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT310190.10100728216948754RNA PCR Primer, Index 1 (100% over 30bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG310100.1009779754368551No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG309070.10064257616339506No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)