FastQCFastQC Report
Thu 24 Aug 2023
SRR10883792_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10883792_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11965174
Total Bases909.3 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG5032414.205881168130108No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG2392891.9998789821192737Illumina Small RNA Adapter 2 (100% over 21bp)
ACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAAC1462841.2225814685185523RNA PCR Primer, Index 1 (100% over 24bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCAA1319381.1026835046443955No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG1280261.0699886186360517Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT1160500.9698981393835142No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAG915370.7650285737591447No Hit
CTGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG809950.6769228763409542No Hit
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC771190.6445288635167362RNA PCR Primer, Index 1 (100% over 24bp)
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA739230.6178180108371177RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC649600.5429089455782257No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA619710.5179281137073309No Hit
CAGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG569390.4758727286372936No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGA520860.4353133518994375RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA496610.4150461999131814No Hit
ACACGGAGTCAAGACCCGCAACGCCCTGGAATTCTCGGGTGCCAAGGAAC470820.3934919793059424RNA PCR Primer, Index 1 (100% over 24bp)
GCTGGTCCGATGGTAGTGGGTTATCTGGAATTCTCGGGTGCCAAGGAACT463750.38758316427324835RNA PCR Primer, Index 1 (100% over 25bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAAC461100.38536840333454403RNA PCR Primer, Index 1 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC454010.3794428731249541No Hit
GTACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTC453270.3788244115798065RNA PCR Primer, Index 1 (100% over 26bp)
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGTGGAATTCTCGGGTGCCAAG440420.36808491042420277No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA440140.36785089794766046No Hit
TTGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG407650.3406970930803012No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGTGGAATTCTCGGGTGCCAA392120.32771775822064936No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG387690.3240153465382116No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTTGGAATTCTCGGGTG368650.3081024981333326No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGG363250.30358940037144466Illumina Small RNA Adapter 2 (100% over 21bp)
CTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGGA342670.2863894833455828RNA PCR Primer, Index 1 (100% over 22bp)
TACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAA340460.2845424562985879RNA PCR Primer, Index 1 (100% over 23bp)
CCGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG334970.2799541402406685No Hit
TAGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG331530.2770791298145769No Hit
ACACGGAGTCAAGACCCGCAACGCCATGGAATTCTCGGGTGCCAAGGAAC305750.2555332667957858RNA PCR Primer, Index 1 (100% over 24bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAG299370.2502011253659997No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCA294370.24602233114202937No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGGA293730.24548744548136114RNA PCR Primer, Index 1 (100% over 22bp)
CTGGTCCGATGGTAGTGGGTTATCTGGAATTCTCGGGTGCCAAGGAACTC292540.2444928924560562RNA PCR Primer, Index 1 (100% over 26bp)
ACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAACTC291750.2438326429686689RNA PCR Primer, Index 1 (100% over 26bp)
AGGATAGCTGGCGCTCTCGCAGACCCGATGGAATTCTCGGGTGCCAAGGA291180.2433562604271363RNA PCR Primer, Index 1 (100% over 22bp)
CAGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA287550.2403224558205338RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG285890.23893509613817568No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA282440.23605172812363615No Hit
CCCACATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAGG273680.22873048064324014Illumina Small RNA Adapter 2 (100% over 21bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG271040.22652407729298377No Hit
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT268170.22412544940842483RNA PCR Primer, Index 1 (100% over 25bp)
TCGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG239650.20028960715489805No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC226770.18952503323395048No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAA220200.18403409762365344No Hit
TAGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA218070.18225393128424208RNA PCR Primer, Index 1 (100% over 22bp)
GTTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAGG217850.1820700643383874Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG217840.18206170674993946No Hit
CTGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA216140.18064091671378954RNA PCR Primer, Index 1 (100% over 22bp)
ACACGGAGTCAAGACCCGCAACGCTTTGGAATTCTCGGGTGCCAAGGAAC208940.17462345303127225RNA PCR Primer, Index 1 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCC206730.17277642598427737No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCTGGAATTCTCGGGTGCCAA206120.172266613088953No Hit
TTGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA196850.16451912859771198RNA PCR Primer, Index 1 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAA189440.15832615555778795RNA PCR Primer, Index 1 (100% over 23bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGCC183620.1534620390810865No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGTGGAATTCTCGGGTGCCA182140.15222511599079128No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG176470.14748636334080892Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG168780.14105937782434255No Hit
CCCGGCTAGCTCAGTCGGTAGAGCATGGTGGAATTCTCGGGTGCCAAGGA157080.13128099934025195RNA PCR Primer, Index 1 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGA157010.1312224962211164RNA PCR Primer, Index 1 (100% over 22bp)
ATGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA156840.13108041721750138RNA PCR Primer, Index 1 (100% over 22bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCTGGAATTCTCGGGTGC152840.12773738183832511No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT150300.1256145543725482No Hit
CGGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG150220.12554769366496465No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGTGGAATTCTCGGGTGCCAAGG147080.1229234108923113Illumina Small RNA Adapter 2 (100% over 21bp)
CCACATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAGGA146030.12204586410527753RNA PCR Primer, Index 1 (100% over 22bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC146020.1220375065168296No Hit
CGGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA137350.11479147733246503RNA PCR Primer, Index 1 (100% over 22bp)
GGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG136900.1144153858523077RNA PCR Primer, Index 4 (100% over 35bp)
AGCAGGGTCGGGCCTGGTTAGTACTTGGTGGAATTCTCGGGTGCCAAGGA133230.11134815089191348RNA PCR Primer, Index 1 (100% over 22bp)
ACACGGAGTCAAGACCCGCAACGCCGTGGAATTCTCGGGTGCCAAGGAAC130890.10939247519509536RNA PCR Primer, Index 1 (100% over 24bp)
GTGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG129650.10835613422755072No Hit
ATTGATCATCGACACTTCGAACGCACTTGGAATTCTCGGGTGCCAAGGAA129040.10784632133222635RNA PCR Primer, Index 1 (100% over 23bp)
ACGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG128900.10772931509395517No Hit
GAGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG128400.10731143567155814No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAGG128300.10722785978707873Illumina Small RNA Adapter 2 (100% over 21bp)
TACACGGAGTCAAGACCCGCAACGCCCTGGAATTCTCGGGTGCCAAGGAA128160.10711085354880756RNA PCR Primer, Index 1 (100% over 23bp)
CCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACT127500.10655925271124349RNA PCR Primer, Index 1 (100% over 25bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCA125380.10478744396028006No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCCAAGGAA122720.10256432543312784RNA PCR Primer, Index 1 (100% over 23bp)
AAGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA121220.10131068716593675RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)