FastQCFastQC Report
Thu 24 Aug 2023
SRR10883793_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10883793_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12065093
Total Bases916.9 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG5134774.255889283240502No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG2448752.029615519747755Illumina Small RNA Adapter 2 (100% over 21bp)
ACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAAC1517861.2580590966020735RNA PCR Primer, Index 1 (100% over 24bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCAA1358531.1260004377919008No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG1315881.0906505237879227Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT1209801.002727455146844No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAG962230.7975321864489565No Hit
CTGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG846010.7012047068348334No Hit
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC805520.6676450815588408RNA PCR Primer, Index 1 (100% over 24bp)
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA764380.6335467119897046RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC662530.5491296254409311No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA619820.5137299811945089No Hit
CAGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG588810.4880277342246761No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGA535630.4439501626717672RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA501080.4153138313977356No Hit
ACACGGAGTCAAGACCCGCAACGCCCTGGAATTCTCGGGTGCCAAGGAAC483700.4009086378364427RNA PCR Primer, Index 1 (100% over 24bp)
GCTGGTCCGATGGTAGTGGGTTATCTGGAATTCTCGGGTGCCAAGGAACT480220.3980242837746879RNA PCR Primer, Index 1 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC472340.3914930452670361No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAAC471220.3905647474080805RNA PCR Primer, Index 1 (100% over 24bp)
GTACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTC470240.3897524867814943RNA PCR Primer, Index 1 (100% over 26bp)
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGTGGAATTCTCGGGTGCCAAG460700.38184537823289055No Hit
TTGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG430950.35718746635438287No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA412860.342193798257502No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGTGGAATTCTCGGGTGCCAA404090.3349248944869302No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG393830.3264210230289978No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTTGGAATTCTCGGGTG375900.31155996891196774No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGG374390.31030842447712587Illumina Small RNA Adapter 2 (100% over 21bp)
CTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGGA359080.2976189242801527RNA PCR Primer, Index 1 (100% over 22bp)
TACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAA352790.2924055371972682RNA PCR Primer, Index 1 (100% over 23bp)
TAGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG352310.2920076952577158No Hit
CCGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG347480.28800441074096983No Hit
ACACGGAGTCAAGACCCGCAACGCCATGGAATTCTCGGGTGCCAAGGAAC314020.26027151220467176RNA PCR Primer, Index 1 (100% over 24bp)
AGGATAGCTGGCGCTCTCGCAGACCCGATGGAATTCTCGGGTGCCAAGGA308670.2558372322534107RNA PCR Primer, Index 1 (100% over 22bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAG307010.2544613622124587No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCA304090.25204115708018165No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGGA303680.25170133375681397RNA PCR Primer, Index 1 (100% over 22bp)
CTGGTCCGATGGTAGTGGGTTATCTGGAATTCTCGGGTGCCAAGGAACTC302240.2505078079381568RNA PCR Primer, Index 1 (100% over 26bp)
ACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAACTC300260.24886670993750315RNA PCR Primer, Index 1 (100% over 26bp)
CAGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA298410.24733336079547832RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG294890.24441585323876075No Hit
CCCACATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAGG285960.2370143354883381Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA279020.23126220411230977No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG276870.2294802037580647No Hit
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT276350.2290492083235496RNA PCR Primer, Index 1 (100% over 25bp)
TCGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG251900.20878413452759959No Hit
TAGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA233080.1931854151476495RNA PCR Primer, Index 1 (100% over 22bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC229050.18984520053015752No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAA228900.1897208749240474No Hit
CTGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA223450.18520371123537963RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG222370.18430856687138675No Hit
GTTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAGG220600.18284152471928727Illumina Small RNA Adapter 2 (100% over 21bp)
ACACGGAGTCAAGACCCGCAACGCTTTGGAATTCTCGGGTGCCAAGGAAC217920.18062024055678644RNA PCR Primer, Index 1 (100% over 24bp)
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCTGGAATTCTCGGGTGCCAA216980.17984113342516297No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCC211830.17557262094871545No Hit
TTGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA208370.17270484363444194RNA PCR Primer, Index 1 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAA198310.16436673965132303RNA PCR Primer, Index 1 (100% over 23bp)
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGTGGAATTCTCGGGTGCCA196350.16274221839815076No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGCC188130.15592917518331603No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG182570.1513208393835008Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG177410.14704403853331258No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGA165930.13752898547901785RNA PCR Primer, Index 1 (100% over 22bp)
ATGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA165880.13748754361031448RNA PCR Primer, Index 1 (100% over 22bp)
CCCGGCTAGCTCAGTCGGTAGAGCATGGTGGAATTCTCGGGTGCCAAGGA164520.1363603247815827RNA PCR Primer, Index 1 (100% over 22bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCTGGAATTCTCGGGTGC157130.13023521658722398No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGTGGAATTCTCGGGTGCCAAGG156290.1295389931930073Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT155990.12929034198078704No Hit
CGGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG154490.12804708591968583No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC149630.12401893628171785No Hit
CCACATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAGGA148800.12333100126124183RNA PCR Primer, Index 1 (100% over 22bp)
CGGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA141840.11756229313773213RNA PCR Primer, Index 1 (100% over 22bp)
GGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG139250.11541560433889736RNA PCR Primer, Index 4 (100% over 35bp)
AGCAGGGTCGGGCCTGGTTAGTACTTGGTGGAATTCTCGGGTGCCAAGGA138150.11450388322742312RNA PCR Primer, Index 1 (100% over 22bp)
GAGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG137520.1139817156817606No Hit
ACGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG136680.1132854922875439No Hit
GTGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG136430.11307828294402705No Hit
TACACGGAGTCAAGACCCGCAACGCCCTGGAATTCTCGGGTGCCAAGGAA136000.11272188287317801RNA PCR Primer, Index 1 (100% over 23bp)
CCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACT134160.11119682210489386RNA PCR Primer, Index 1 (100% over 25bp)
ATTGATCATCGACACTTCGAACGCACTTGGAATTCTCGGGTGCCAAGGAA132700.10998671953875531RNA PCR Primer, Index 1 (100% over 23bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCCAAGGAA129700.10750020741655286RNA PCR Primer, Index 1 (100% over 23bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAGG128640.10662163980004133Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCA128440.10645587232522784No Hit
ACACGGAGTCAAGACCCGCAACGCCGTGGAATTCTCGGGTGCCAAGGAAC127020.105278923254052RNA PCR Primer, Index 1 (100% over 24bp)
AAGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA126240.10463243010227936RNA PCR Primer, Index 1 (100% over 22bp)
AAATCAGTTATGGTTCCTTTGGTCGCTCTGGAATTCTCGGGTGCCAAGGA125220.10378701598073052RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGTGGAATTCTCGGGTGCCA121990.10110987126249255No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)