FastQCFastQC Report
Thu 24 Aug 2023
SRR10883794_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10883794_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12479022
Total Bases948.4 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG5284974.235083486510401No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG2500842.0040352521215206Illumina Small RNA Adapter 2 (100% over 21bp)
ACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAAC1567531.2561320911206022RNA PCR Primer, Index 1 (100% over 24bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCAA1393221.1164496704950115No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG1330141.065900837421394Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT1236050.9905023005809268No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAG975150.7814314294822142No Hit
CTGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG868720.6961442972053419No Hit
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC815340.6533685091668242RNA PCR Primer, Index 1 (100% over 24bp)
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA781310.6260987439560568RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC687350.5508043819459569No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA646040.51770082623462No Hit
CAGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG600890.4815201063032023No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGA548990.439930308641174RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA522550.41874275083415996No Hit
ACACGGAGTCAAGACCCGCAACGCCCTGGAATTCTCGGGTGCCAAGGAAC500680.40121733898698153RNA PCR Primer, Index 1 (100% over 24bp)
GCTGGTCCGATGGTAGTGGGTTATCTGGAATTCTCGGGTGCCAAGGAACT492750.3948626743345752RNA PCR Primer, Index 1 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC480180.38478976958290484No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAAC478170.38317906643645633RNA PCR Primer, Index 1 (100% over 24bp)
GTACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTC477240.3824338157269055RNA PCR Primer, Index 1 (100% over 26bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA471190.37758567939058046No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGTGGAATTCTCGGGTGCCAAG466810.3740757889520509No Hit
TTGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG433160.3471105347838957No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGTGGAATTCTCGGGTGCCAA416170.33349568579973654No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG401970.3221165889442298No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTTGGAATTCTCGGGTG388580.31138658141639625No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGG380490.30490370158815333Illumina Small RNA Adapter 2 (100% over 21bp)
CTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGGA364730.29227450676823874RNA PCR Primer, Index 1 (100% over 22bp)
TACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAA362720.29066380362179023RNA PCR Primer, Index 1 (100% over 23bp)
CCGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG354980.2844613944906901No Hit
TAGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG354550.2841168162056289No Hit
ACACGGAGTCAAGACCCGCAACGCCATGGAATTCTCGGGTGCCAAGGAAC324850.26031687419094224RNA PCR Primer, Index 1 (100% over 24bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAG314860.2523114391496385No Hit
ACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAACTC313240.25101326049429196RNA PCR Primer, Index 1 (100% over 26bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCA310760.24902592526882314No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGGA309500.24801623075910917RNA PCR Primer, Index 1 (100% over 22bp)
AGGATAGCTGGCGCTCTCGCAGACCCGATGGAATTCTCGGGTGCCAAGGA306860.24590068035780366RNA PCR Primer, Index 1 (100% over 22bp)
CAGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA305960.24517946999372225RNA PCR Primer, Index 1 (100% over 22bp)
CTGGTCCGATGGTAGTGGGTTATCTGGAATTCTCGGGTGCCAAGGAACTC303230.24299179855600864RNA PCR Primer, Index 1 (100% over 26bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA299390.23991463433592794No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG298450.23916137017788733No Hit
CCCACATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAGG291240.2333836738167462Illumina Small RNA Adapter 2 (100% over 21bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG287830.2306510878817266No Hit
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT279220.2237515087320144RNA PCR Primer, Index 1 (100% over 25bp)
TCGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG257940.2066988903457338No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC237540.19035145542655507No Hit
TAGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA235920.18905327677120853RNA PCR Primer, Index 1 (100% over 22bp)
AACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAA235810.1889651288378208No Hit
GTTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAGG228430.18305120385235316Illumina Small RNA Adapter 2 (100% over 21bp)
CTGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA227720.18248224900957785RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG226810.18175302519700662No Hit
ACACGGAGTCAAGACCCGCAACGCTTTGGAATTCTCGGGTGCCAAGGAAC226020.18111996276631295RNA PCR Primer, Index 1 (100% over 24bp)
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCTGGAATTCTCGGGTGCCAA222100.1779786909583139No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCC217780.1745168812107231No Hit
TTGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA206070.16513313302917487RNA PCR Primer, Index 1 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAA200740.16086196498411492RNA PCR Primer, Index 1 (100% over 23bp)
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGTGGAATTCTCGGGTGCCA197190.15801719077023824No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGCC189080.15151828404501572No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG186160.149178357085996Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG182110.14593291044762963No Hit
ATGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA169110.13551542741089806RNA PCR Primer, Index 1 (100% over 22bp)
CCCGGCTAGCTCAGTCGGTAGAGCATGGTGGAATTCTCGGGTGCCAAGGA166410.13335179631865382RNA PCR Primer, Index 1 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGA165370.1325183976757153RNA PCR Primer, Index 1 (100% over 22bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCTGGAATTCTCGGGTGC159470.12779046306673714No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGTGGAATTCTCGGGTGCCAAGG158940.12736575029677805Illumina Small RNA Adapter 2 (100% over 21bp)
CGGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG158930.12735773684828827No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT158610.12710130649661489No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC153890.12331895880943235No Hit
CCACATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAGGA151770.12162010772959611RNA PCR Primer, Index 1 (100% over 22bp)
CGGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA145750.11679601173874042RNA PCR Primer, Index 1 (100% over 22bp)
AGCAGGGTCGGGCCTGGTTAGTACTTGGTGGAATTCTCGGGTGCCAAGGA141770.11360665923980261RNA PCR Primer, Index 1 (100% over 22bp)
ACGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG139910.11211615782070102No Hit
GGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG139800.11202800988731328RNA PCR Primer, Index 4 (100% over 35bp)
GTGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG137930.1105294950197219No Hit
GAGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG137170.1099204729344976No Hit
ATTGATCATCGACACTTCGAACGCACTTGGAATTCTCGGGTGCCAAGGAA136630.10948774671604873RNA PCR Primer, Index 1 (100% over 23bp)
CCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACT136020.10899892635817132RNA PCR Primer, Index 1 (100% over 25bp)
TACACGGAGTCAAGACCCGCAACGCCCTGGAATTCTCGGGTGCCAAGGAA135890.108894751527804RNA PCR Primer, Index 1 (100% over 23bp)
ACACGGAGTCAAGACCCGCAACGCCGTGGAATTCTCGGGTGCCAAGGAAC133470.10695549699327399RNA PCR Primer, Index 1 (100% over 24bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCA133230.10676317422951893No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAGG132500.10617819248976401Illumina Small RNA Adapter 2 (100% over 21bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCCAAGGAA131490.10536883419229488RNA PCR Primer, Index 1 (100% over 23bp)
AAGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA130240.1043671531310707RNA PCR Primer, Index 1 (100% over 22bp)
AAATCAGTTATGGTTCCTTTGGTCGCTCTGGAATTCTCGGGTGCCAAGGA125990.10096143752290844RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)