FastQCFastQC Report
Thu 24 Aug 2023
SRR10883795_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10883795_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12283190
Total Bases933.5 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG5182704.2193436721242605No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG2459132.0020287889383783Illumina Small RNA Adapter 2 (100% over 21bp)
ACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAAC1569481.2777462532127242RNA PCR Primer, Index 1 (100% over 24bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCAA1366191.112243643548622No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG1324131.0780017243077735Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT1242851.011829988789557No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAG989140.8052794103160498No Hit
CTGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG871420.7094411142382394No Hit
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC817690.6656984057073122RNA PCR Primer, Index 1 (100% over 24bp)
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA767230.6246178720674352RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC669940.5454120631529757No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA616080.501563518923016No Hit
CAGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG602460.49047519414744867No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGA542030.4412778765125346RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA508150.4136954651031206No Hit
ACACGGAGTCAAGACCCGCAACGCCCTGGAATTCTCGGGTGCCAAGGAAC503190.40965742612464673RNA PCR Primer, Index 1 (100% over 24bp)
GCTGGTCCGATGGTAGTGGGTTATCTGGAATTCTCGGGTGCCAAGGAACT487640.39699784827882656RNA PCR Primer, Index 1 (100% over 25bp)
GTACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTC484080.39409957836685744RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC472480.38465577753010416No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAAC470280.3828647118541682RNA PCR Primer, Index 1 (100% over 24bp)
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGTGGAATTCTCGGGTGCCAAG466060.37942912223941827No Hit
TTGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG441250.3592307861394312No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA425340.34627812481936693No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGTGGAATTCTCGGGTGCCAA406490.33093194845964286No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG386500.31465767443147913No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTTGGAATTCTCGGGTG381850.3108720128891599No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGG381200.310342834393997Illumina Small RNA Adapter 2 (100% over 21bp)
CTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGGA365270.29737389065869696RNA PCR Primer, Index 1 (100% over 22bp)
TAGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG359160.2923996128041657No Hit
CCGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG358360.2917483161947344No Hit
TACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAA353950.2881580436352446RNA PCR Primer, Index 1 (100% over 23bp)
ACACGGAGTCAAGACCCGCAACGCCATGGAATTCTCGGGTGCCAAGGAAC322670.2626923462064822RNA PCR Primer, Index 1 (100% over 24bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAG318340.2591672033079355No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGGA313680.2553734005579984RNA PCR Primer, Index 1 (100% over 22bp)
CAGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA308160.25087945395292266RNA PCR Primer, Index 1 (100% over 22bp)
AGGATAGCTGGCGCTCTCGCAGACCCGATGGAATTCTCGGGTGCCAAGGA307550.2503828402882313RNA PCR Primer, Index 1 (100% over 22bp)
ACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAACTC307500.2503421342501419RNA PCR Primer, Index 1 (100% over 26bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCA304780.24812772577807557No Hit
CTGGTCCGATGGTAGTGGGTTATCTGGAATTCTCGGGTGCCAAGGAACTC303890.24740315830008328RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG290930.2368521532272968No Hit
CCCACATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAGG289270.23550071276272697Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA284430.23156036827566778No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG281150.22889005217699962No Hit
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT281080.22883306372367437RNA PCR Primer, Index 1 (100% over 25bp)
TCGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG261850.21317752147447042No Hit
TAGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA238530.19419222530954908RNA PCR Primer, Index 1 (100% over 22bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC230640.18776881249903324No Hit
CTGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA230370.1875489998933502RNA PCR Primer, Index 1 (100% over 22bp)
AACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAA230240.1874431641943176No Hit
GTTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAGG228430.18596960561547937Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG226680.1845448942823485No Hit
ACACGGAGTCAAGACCCGCAACGCTTTGGAATTCTCGGGTGCCAAGGAAC223980.18234676822551796RNA PCR Primer, Index 1 (100% over 24bp)
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCTGGAATTCTCGGGTGCCAA222140.18084878602382606No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCC213050.17344842829916332No Hit
TTGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA210420.17130729069565806RNA PCR Primer, Index 1 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAA200120.16292184684923053RNA PCR Primer, Index 1 (100% over 23bp)
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGTGGAATTCTCGGGTGCCA197690.16094353339808307No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGCC186480.1518172396584275No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG184890.15052278764718285Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG182710.14874800438648267No Hit
CCCGGCTAGCTCAGTCGGTAGAGCATGGTGGAATTCTCGGGTGCCAAGGA167080.13602329687971937RNA PCR Primer, Index 1 (100% over 22bp)
ATGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA166710.1357220721978574RNA PCR Primer, Index 1 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGA166100.13522545853316606RNA PCR Primer, Index 1 (100% over 22bp)
AAATCAGTTATGGTTCCTTTGGTCGCTCGTGGAATTCTCGGGTGCCAAGG160690.13082106521188713Illumina Small RNA Adapter 2 (100% over 21bp)
CGGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG157690.1283787029265199No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT154370.12567582199738014No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCTGGAATTCTCGGGTGC154000.1253745973155182No Hit
CCACATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAGGA147890.12040031946098692RNA PCR Primer, Index 1 (100% over 22bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC146340.11913843228021385No Hit
CGGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA145310.1182998878955711RNA PCR Primer, Index 1 (100% over 22bp)
AGCAGGGTCGGGCCTGGTTAGTACTTGGTGGAATTCTCGGGTGCCAAGGA140710.11455493239134133RNA PCR Primer, Index 1 (100% over 22bp)
GAGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG140140.11409088355712156No Hit
GTGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG139080.11322791554962515No Hit
GGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG138620.11285341999920218RNA PCR Primer, Index 4 (100% over 35bp)
ACGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG138000.11234866512689293No Hit
ATTGATCATCGACACTTCGAACGCACTTGGAATTCTCGGGTGCCAAGGAA137600.11202301682217729RNA PCR Primer, Index 1 (100% over 23bp)
CCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACT136000.11072042360331477RNA PCR Primer, Index 1 (100% over 25bp)
TACACGGAGTCAAGACCCGCAACGCCCTGGAATTCTCGGGTGCCAAGGAA134060.10914102932544395RNA PCR Primer, Index 1 (100% over 23bp)
AAGTACACGGAGTCGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGA132140.10757791746280894RNA PCR Primer, Index 1 (100% over 22bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCCAAGGAA128730.10480176566510817RNA PCR Primer, Index 1 (100% over 23bp)
ACACGGAGTCAAGACCCGCAACGCCGTGGAATTCTCGGGTGCCAAGGAAC128510.10462265909751457RNA PCR Primer, Index 1 (100% over 24bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAGG128290.10444355252992098Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCA126740.10318166534914791No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCTGGAATTCTCGGGTGCCAAGGA126560.10303512361202587RNA PCR Primer, Index 1 (100% over 22bp)
AGGATAGCTGGCGCTCTCGCAGACCCGTGGAATTCTCGGGTGCCAAGGAA123370.1004380783819187RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)