FastQCFastQC Report
Thu 24 Aug 2023
SRR10883798_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10883798_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16612442
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG9998196.018495053285965No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG4824522.9041606285216828Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG2552991.5367939283098775Illumina Small RNA Adapter 2 (100% over 21bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCAA2292851.3802004545749504No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA1672931.0070343661696457No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAG1481450.8917713602852608No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA1410160.8488577416854187RNA PCR Primer, Index 1 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC1086030.6537449461072611RNA PCR Primer, Index 1 (100% over 24bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA989160.5954332301054837No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGA982550.5914542846861407RNA PCR Primer, Index 1 (100% over 22bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG933260.5617837522021145No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCA882460.5312042624437756No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCC832790.5013049857450217No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTTGGAATTCTCGGGTG721460.4342889504143942No Hit
GCTGGTCCGATGGTAGTGGGTTATCTGGAATTCTCGGGTGCCAAGGAACT679280.408898342579616RNA PCR Primer, Index 1 (100% over 25bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT608700.3664121144862387No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGTGGAATTCTCGGGTGCCAA594340.35776799100336965No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGTGGAATTCTCGGGTGCCAAG581530.3500569031332058No Hit
CTGGTCCGATGGTAGTGGGTTATCTGGAATTCTCGGGTGCCAAGGAACTC562120.3383728894282972RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG541620.3260327410021958No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG533890.32137960210786587No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGGA432680.26045538639051385RNA PCR Primer, Index 1 (100% over 22bp)
CCCACATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAGG429040.25826425759680605Illumina Small RNA Adapter 2 (100% over 21bp)
CTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGGA417010.2510226973252939RNA PCR Primer, Index 1 (100% over 22bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGG407180.24510544566536335Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG388630.23393911623589117Illumina Small RNA Adapter 2 (100% over 21bp)
ACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAAC325140.19572077362256554RNA PCR Primer, Index 1 (100% over 24bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGTGGAATTCTCGGGTGCCA309990.18660110295644672No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC307100.18486144300759635No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAG305190.1837117023493596No Hit
GTTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAGG293050.17640392664726837Illumina Small RNA Adapter 2 (100% over 21bp)
CCCGGCTAGCTCAGTCGGTAGAGCATGGTGGAATTCTCGGGTGCCAAGGA267710.16115029927568747RNA PCR Primer, Index 1 (100% over 22bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAGG255140.153583681435878Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCA236390.14229696031444383No Hit
CTGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG234540.14118333716379566No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCTGGAATTCTCGGGTGCCAA231180.13916075673883466No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAAG225250.1355911430721624No Hit
CCACATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAGGA217580.13097412168542108RNA PCR Primer, Index 1 (100% over 22bp)
CCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACT208970.12579125934645852RNA PCR Primer, Index 1 (100% over 25bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCTGGAATTCTCGGGTGC207090.12465957744201604No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAA204480.12308846586191241RNA PCR Primer, Index 1 (100% over 23bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGCC198960.1197656551637622No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC195670.11778521183098789No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG186500.11226525275453182No Hit
CCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAA180230.10849097321152423No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAAC177410.10679345035486054RNA PCR Primer, Index 1 (100% over 24bp)
CCCTGGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACT170710.10276032867413472RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)