FastQCFastQC Report
Thu 24 Aug 2023
SRR10883799_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10883799_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16243209
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG9794026.029609050773157No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG4739712.917964055009081Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG2526001.5551114314911543Illumina Small RNA Adapter 2 (100% over 21bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCAA2242371.380496920282193No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA1593700.9811484910401633No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAG1479290.9107129016193782No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA1382660.8512234251249245RNA PCR Primer, Index 1 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC1087390.6694428422364078RNA PCR Primer, Index 1 (100% over 24bp)
CCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGA973090.5990749734242784RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA941070.5793621198865322No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG879960.5417402435688662No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCA867680.5341801610753146No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCC810630.4990577908589368No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTTGGAATTCTCGGGTG710680.43752438326687787No Hit
GCTGGTCCGATGGTAGTGGGTTATCTGGAATTCTCGGGTGCCAAGGAACT676250.41632783275767743RNA PCR Primer, Index 1 (100% over 25bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT613590.37775171149986436No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGTGGAATTCTCGGGTGCCAAG572740.3526027400127647No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGTGGAATTCTCGGGTGCCAA565920.348404062276118No Hit
CTGGTCCGATGGTAGTGGGTTATCTGGAATTCTCGGGTGCCAAGGAACTC560880.34530122711589806RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG527240.32459103370522413No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG514060.3164768735044904No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGGA436390.26865996737467335RNA PCR Primer, Index 1 (100% over 22bp)
CCCACATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAGG422780.2602810811582859Illumina Small RNA Adapter 2 (100% over 21bp)
CTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGGA419350.25816942945202515RNA PCR Primer, Index 1 (100% over 22bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGG410110.25248089832495535Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG380430.23420864682588272Illumina Small RNA Adapter 2 (100% over 21bp)
ACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAGGAAC324460.1997511698581235RNA PCR Primer, Index 1 (100% over 24bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAG298630.1838491396619966No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGTGGAATTCTCGGGTGCCA294860.18152816971080038No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC293110.1804507963912796No Hit
GTTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAGG289700.1783514575229562Illumina Small RNA Adapter 2 (100% over 21bp)
CCCGGCTAGCTCAGTCGGTAGAGCATGGTGGAATTCTCGGGTGCCAAGGA263950.16249867867857884RNA PCR Primer, Index 1 (100% over 22bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAGG243520.14992111472554467Illumina Small RNA Adapter 2 (100% over 21bp)
CTGTACACGGAGTCAAGACCCGCAACGCCTTGGAATTCTCGGGTGCCAAG234630.14444805826237908No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAAG229570.14133291026422182No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCA228150.1404586987706678No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCTGGAATTCTCGGGTGCCAA225860.13904887882683772No Hit
CCACATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAGGA208780.1285337152283148RNA PCR Primer, Index 1 (100% over 22bp)
CCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACT207730.1278872912366023RNA PCR Primer, Index 1 (100% over 25bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAA204330.12579410878724764RNA PCR Primer, Index 1 (100% over 23bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCTGGAATTCTCGGGTGC194530.1197608181979312No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGCC192590.1185664729180053No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC188730.11619009519609087No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG184680.11369674551377133No Hit
CCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAA180440.11108642387104666No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAAC168610.10380338023108611RNA PCR Primer, Index 1 (100% over 24bp)
CCCTGGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACT165900.10213499069057107RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)