FastQCFastQC Report
Thu 24 Aug 2023
SRR10887268.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10887268.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37894181
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length39
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC10733122.832392656803956RNA PCR Primer, Index 1 (95% over 22bp)
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGG1248970.32959414006071275No Hit
CTTCTGGCGCCAAGCGTCCGTCCCTGGAATTCTCGGGTG525580.13869675663395392No Hit
CTTCTGGCGCCAAGCGTCCGTCCCGCTGGAATTCTCGGG523690.1381979993181539No Hit
ACCCCGAGTGTTACAGCCCTCCGGCTGGAATTCTCGGGT482300.1272754779948932No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAA476100.1256393428848614No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)