FastQCFastQC Report
Thu 24 Aug 2023
SRR10887271.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10887271.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46905207
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length39
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGG12113392.58252564582009No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAA6822971.454629546779316No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTG3012520.642257052612517No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAA2532830.5399890890578524No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTG2361800.503526186335773No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGA2227320.4748555954565982No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAA2216300.4725061761266718No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTTGGAATT2148820.4581197136599355No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGG1959040.41765938694183785No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAA1633880.34833659299275666No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTTGGA1587610.33847201655031606No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAAT1547990.33002519315179657No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTGGAATTC1529570.3260981238181083No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGG1526380.32541802874891906No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAAT1499630.3197150371812665No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAAT1427510.30433934552298214No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATT1268240.2703836271312053No Hit
CGGCGGATCTTTCCCGCTCCCCGCCTCCCGACCCCTTGG1230090.2622502017739736No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGGAATTCT1213370.2586855655492577No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAA1196900.25517422831115527No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAA1171720.24980595437943598No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTTGGAAT1142330.243540125513144No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTCTGG1081420.23055436041461239No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGTGGAATTCTC1079270.2300959891297356No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGGAA1014360.21625744024538684No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTG949470.2024231552799671No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATT936330.19962176054355757No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGA931420.1985749684464669No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGGAAT909200.19383775451625232No Hit
TTCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTTGG831280.17722552636853303No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGG817680.1743260614967545No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCT815660.17389540568491682No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTGGAATT801890.17095969750224105No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGTG704210.15013471745258475No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCT698040.14881929846296169No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATT697800.14876813143581266No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAAT693510.1478535208255237No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTG683830.14578978406384605No Hit
ACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAATTC634820.13534105072812067No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGGAAT628360.13396380491402585No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGG612440.13056972544647336No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGG563580.1201529715027161No Hit
TTCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTCTG549870.11723005507682761No Hit
TGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC547010.1166203146699683RNA PCR Primer, Index 1 (95% over 22bp)
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTC542320.11562042568109762No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGTGGAATT509210.10856150789399564No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGGAATTC503070.10725248478276622No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGTGGAA500540.10671309903823685No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCTGGAAT487400.10391170430182729No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGTGGA473280.10090137753789254No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)