FastQCFastQC Report
Thu 24 Aug 2023
SRR10887272.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10887272.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45094438
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length39
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAA4501420.9982206674801004No Hit
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGG3301240.732072545177301No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTG3139540.696214464409114No Hit
TGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC2776260.6156546401576176RNA PCR Primer, Index 1 (95% over 22bp)
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTG2226470.493734947977398No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAA2165890.4803009187075355No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCT1874690.4157253273674239No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAAT1360820.30177114082228945No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTTGGAATT1356190.30074440666052876No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAAT1314570.29151488704660206No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGA1303520.2890644739823568No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGGAATTCT1192060.2643474567750462No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAA1180840.261859345048274No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAA1160310.2573066771560608No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTTGGAAT1156560.25647508901208615No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGG1132890.25122610464731815No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAAT1043770.23146313520971254No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATT985660.2185768453306814No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGG918880.20376792366278076No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCT878610.19483777578068495No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAA876800.19443639590319323No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTTGGA810940.17983149052661437No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTG736280.16327512497217506No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTCTGG693160.15371297010065854No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGTGGAATTCTC687510.15246004396373672No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGG663580.1471534028209865No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGG643210.14263621602291618No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATT640800.14210178204238846No Hit
TTCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTTGG618060.13705903153732618No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGTG604490.13404979124032992No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTGGAATTC600980.13327142473756964No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGGAAT566260.12557202730855632No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAA539650.11967107783891219No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGGAAT533030.11820304756874896No Hit
CGGCGGATCTTTCCCGCTCCCCGCCTCCCGACCCCTTGG528190.11712974447092565No Hit
TTCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTCTG518700.1150252720745738No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTGGAATT500550.11100038545773648No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGGAA493060.10933942673817112No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGG465310.1031856744727587No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTG462760.10262019453485595No Hit
ACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAATTC458450.10166442256138107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)