FastQCFastQC Report
Thu 24 Aug 2023
SRR10887273.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10887273.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51808482
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length39
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGG6830711.3184539936916122No Hit
TGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC5203271.004327824158214RNA PCR Primer, Index 1 (95% over 22bp)
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAA1646430.3177915925041No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTG1585310.30599429645516346No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCT1513130.29206221483192657No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTG1454630.28077062748142284No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAAT867260.16739729992474978No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGG616840.11906158532110629No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAA609230.11759271387260486No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAA602920.11637476658744798No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATT582080.11235225923044803No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTG551220.10639570562982331No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAA519100.10019594860934161No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)