FastQCFastQC Report
Thu 24 Aug 2023
SRR10887274.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10887274.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61333869
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length39
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAA14626252.3846938467227625No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTG9404861.5333876948150784No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTG5595350.9122773585341568No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAA5070380.8266851712876616No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGG4563970.7441190445689966No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAA4124190.6724164099284199No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAA3816440.622240217717229No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGA3631860.5921459153343156No Hit
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGG3584930.5844943517259608No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCT3543990.5778194100228701No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTG3226190.5260046451659524No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGG3075110.5013722516021286No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGGAATTCT3033500.4945880717226562No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAAT2717970.4431434123290021No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATT2522980.4113518421608133No Hit
TGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC2198770.35849197773582486RNA PCR Primer, Index 1 (95% over 22bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAAT2010150.32773898545353464No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGG1926040.3140255182662617No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGG1912470.31181303758939455No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAAT1855870.30258485731594725No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAA1783560.2907952863694283No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGG1562250.2547124493320322No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAA1390370.22668878103874385No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGTGGAATTCTC1381200.22519368540080195No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCTGGAAT1324600.21596550512735468No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGG1307160.21312205170034193No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGG1289800.21029164163767333No Hit
GCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTG1253020.20429495488047555No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGGAAT1179940.19237984155214471No Hit
TAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGA1148740.18729292945794762No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGTG1122920.18308318361589093No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGCCTTGGA1093420.17827344301400586No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTG1007800.16431378232473806No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCT1000230.16307955397367807No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGGAA998930.16286759930308653No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGGAA966660.15760623221078718No Hit
TTCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTCTG960710.1566361319876951No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGGAA954290.15558940199908144No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGA954120.15556168484985025No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGA944950.15406658921190836No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGGAATTC915220.14921934893753402No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTTGGAAT892830.1455688373417304No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTCTGG803970.13108092039652675No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGGAAT798630.13021027582655842No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCAGTGGA789120.12865974589015408No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGTGGAATTCTCG760220.12394783052084973No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGGA741410.12088100947944438No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGA726390.11843211782384053No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATT708470.11551040421076322No Hit
TTCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTTGG707610.11537018804406421No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAATT696300.11352618240991776No Hit
ACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAATTC695490.11339411834593378No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTTGGAATT648770.10577679356898226No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGGAAT631270.10292355761871146No Hit
GGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTG628760.10251432206241547No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGG626760.1021882379538131No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGTGGAATT620770.10121161604854896No Hit
GCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGG617460.10067194684881203No Hit
TAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAA616140.10045673133713445No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)