FastQCFastQC Report
Thu 24 Aug 2023
SRR10887275.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10887275.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61659978
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length39
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGG22940013.7204051548639865No Hit
TGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC10204691.654994103306362RNA PCR Primer, Index 1 (95% over 22bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAA4308330.698723895100968No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGGAATTCT2800480.4541811545894486No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGTGGAATTCTC1903330.3086815892149686No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAA1468750.23820151217050387No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTG1153710.18710840279573243No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAAT1090170.17680350129219963No Hit
TGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGT970120.15733382194849307No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGGAATTC887160.14387938964233818No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCTGGAAT864670.14023196699810694No Hit
ACGGAGTCGAGCTCAACCCGCAACGCGATGGAATTCTCG799190.12961243677381784No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGGAAT790010.1281236266415794No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTG787670.12774412601963628No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGGAA655230.10626503953666672No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGTGGAATT638020.10347392598810204No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)