FastQCFastQC Report
Thu 24 Aug 2023
SRR10887276.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10887276.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60146348
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length39
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC10707911.7803092550191075RNA PCR Primer, Index 1 (95% over 22bp)
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAA4021800.6686690270870644No Hit
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGG3565960.592880551949721No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTG3194380.5311012399289813No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTG2425230.40322148902540184No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAA1880000.3125709311561194No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCT1701990.28297478676510834No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAA1370340.2278342818087642No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGG1323570.22005824859058776No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAA1153630.1918038315476777No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTG1150950.19135825170964663No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAA1058720.17602398735830146No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGA1041070.173089478350373No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGGAATTCT959360.15950428112443335No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGG800740.13313194011380375No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAAT665610.1106650731312897No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATT644690.1071868902165099No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAAT629920.10473121327333125No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)