FastQCFastQC Report
Wed 30 Aug 2023
SRR10887277.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10887277.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73029074
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length39
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTGGAATTC13170531.8034639190413395No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTGGAATT5541250.7587731428718376No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATT3282820.4495223368161562No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAAT3140890.43008761140802637No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTGGAATT2765170.37863960865777924No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGGAATTCT2446870.3350542278545118No Hit
CGGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTC2129760.29163179585160837No Hit
GGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTCT2105920.28836734257372615No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTTGGAATT2105190.28826738238526756No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGTGGAATTCTC1961960.26865464568262226No Hit
GGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTCTC1867400.25570637798310303No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGTGGAATTCTCG1785110.244438263040279No Hit
CCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTG1686380.2309189898806604No Hit
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTGGAATT1644890.2252376909503193No Hit
CTTCTGGCGCCAAGCGTCCGTCCCGCGTGGAATTCTCGG1627100.22280167485075877No Hit
CGGGGGCCCGAGGTGGGATCCCGAGGCCTGGAATTCTCG1606550.21998772707976555No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGGAATTCT1534710.2101505490813152No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCT1432420.19614379883825445No Hit
TGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC1418390.1942226461751384RNA PCR Primer, Index 1 (95% over 22bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATT1372390.18792378498459394No Hit
TCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATT1316410.18025834477923136No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGTGGAATT1302310.17832760689256447No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTGGAATTCTC1225530.16781398597495567No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAAT1224210.16763323604514005No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATT1222720.1674292077152724No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATT1196960.1639018454485675No Hit
GGGGCGGGGGGCCCGGACACTGGAATTCTCGGGTGCCAA1182770.16195878370304956No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATT1115150.15269945775294919No Hit
CGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGC1098950.15048116315975743No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGGAATTC1076090.14735090301158685No Hit
TCGGGGGCCCGAGGTGGGATCCCGAGGCCTGGAATTCTC1073470.14699214178725584No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGGAATTC1029710.14100000775033789No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGTGGAATTCTC1025840.14047008181974208No Hit
CGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTG994120.13612660623356665No Hit
TGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTCT977280.13382067531076733No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTTGGAAT897360.12287708865102137No Hit
CCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGT876690.12004670906822672No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTC808110.11065592862371498No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTATGGAATTCTCGG806450.11042862189379533No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTGGAAT783140.10723674245136944No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCTGGAATTCTCGGGT766170.10491301039911857No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGTGGAATTCTC745120.10203059674561941No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTC742560.10168005142718911No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGGAATTCTCG738640.10114327890834274No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)