FastQCFastQC Report
Thu 24 Aug 2023
SRR10887279.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10887279.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63108502
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length39
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAAT14215822.2525998161071863No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAA10536331.6695579305621926No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATT9188961.4560573787664934No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAA4261920.6753321446292609No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTGGAATT3007320.47653167238861094No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAA3000440.4754414864735658No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAA2740070.43418397096479966No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAAT2664100.42214597329532555No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGGAATTCT2536470.4019220738277071No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGA2491600.39481209679164936No Hit
TGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC2445070.38743908071213606RNA PCR Primer, Index 1 (95% over 22bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTGGAATTC2107960.33402155544747364No Hit
CGGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTC1956230.30997883613209515No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGGAAT1943220.3079173072433251No Hit
CGGCGGATCTTTCCCGCTCCCCGCCTCCCGACCCCTTGG1939460.30732150796417257No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAAT1929820.3057939800250686No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAA1800790.2853482404003188No Hit
CGGGGGCCCGAGGTGGGATCCCGAGGCCTGGAATTCTCG1697060.26891146932944154No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTTGGAAT1683420.26675011236996243No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAAT1669030.264469912469163No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATT1653790.2620550239015339No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGCCTTGGA1646740.2609379002531228No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTTGGAATT1641150.2600521241971486No Hit
TCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATT1481200.23470688624489933No Hit
TCGGGGGCCCGAGGTGGGATCCCGAGGCCTGGAATTCTC1333420.21129007308714123No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGG1285490.20369521685049663No Hit
GGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTCT1276350.2022469175389395No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAA1272460.20163051881662472No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCTGGAAT1259780.19962128082203567No Hit
GGGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTC1258710.19945173155908533No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTGGAATTCTC1190540.1886497004793427No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTG1090540.17280397496996522No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTGGAAT1070740.16966652131910848No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTGGAATT1060730.16808036419561978No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTC1035730.16411893281827541No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATT1031530.16345341234688157No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTC1020960.16177851916054037No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGTGGAATTCTCG933630.147940447073201No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGTGGAATTCTC923360.14631309106338794No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGGAATTCT910970.14434980567277605No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGTGGAATT876260.13884975434847116No Hit
GGGGGGCCCGAGGTGGGATCCCGAGGCCTCTGGAATTCT864770.13702908048744367No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTTGGAAT857710.13591037226648162No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGGAATTC857650.135900864831176No Hit
ACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAATTC843800.13370623184812722No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATT772230.12236544610106574No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTCTGG757020.11995531125108944No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTTGGA752530.11924383817571839No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGTGGAATTCTC723160.11458994859361421No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAAT709540.112431760779237No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAATT665540.10545964155511091No Hit
GGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTCTC663060.10506666756247836No Hit
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGG656690.104057294847531No Hit
CTTCTGGCGCCAAGCGTCCGTCCCGCGTGGAATTCTCGG645710.10231743418660136No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)