FastQCFastQC Report
Thu 24 Aug 2023
SRR10887280.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10887280.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60803337
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length39
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAAT12827272.1096325683572266No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAA7018541.1543017778777505No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATT6881611.131781632314029No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAA3537340.5817674118774107No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTG3380060.5559004105317443No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGGAATTCT2577800.4239569943340445No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAA2491080.4096946192278888No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAA2449030.40277888037625303No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGA2263420.37225259528107807No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAAT1987020.32679456392335837No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAAT1806050.29703139483939833No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAAT1650000.2713666850225671No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGGAAT1559620.256502369269634No Hit
CGGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTC1542400.253670287865944No Hit
TCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATT1531050.2518036140023039No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCTGGAAT1461780.2404111471710837No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTTGGAAT1422880.2340134719908547No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATT1415400.23278327635208573No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAA1407670.23151196454891942No Hit
CGGGGGCCCGAGGTGGGATCCCGAGGCCTGGAATTCTCG1297260.21335342170446994No Hit
TCGGGGGCCCGAGGTGGGATCCCGAGGCCTGGAATTCTC1275330.20974671176353363No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGG1239970.20393124147117123No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTGGAATTCTC1230500.20237376116379927No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCT1090950.1794227181971937No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGG945620.15552106950972117No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATT864650.14220436618470464No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGCCTTGGA806870.1327015982691871No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTC785000.12910475620770615No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGTGGAATTCTC781070.1284584101033797No Hit
GGGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTC751800.12364452957573695No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGGAATTC717840.11805930980399973No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCT717040.11792773807792818No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAAT713700.11737842612157949No Hit
TGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC696010.11446904632882238RNA PCR Primer, Index 1 (95% over 22bp)
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCT665050.10937722052985349No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAAT639160.10511923054486302No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATT630350.10367029691150009No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTGGAATT630120.10363247004025454No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTTGGAA619770.10193026083420388No Hit
CGGGCGGGCGTCCCGCGCGTGTGGGGGGAACTGGAATTC613630.10092044783660475No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)