FastQCFastQC Report
Thu 24 Aug 2023
SRR10887281.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10887281.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55937482
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length39
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAAT22862934.0872290247172725No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATT17763353.1755719715807014No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAA5873511.050013298775229No Hit
CGGGGGCCCGAGGTGGGATCCCGAGGCCTGGAATTCTCG4533350.8104315457031118No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTG3379140.6040922614285712No Hit
CGGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTC3118750.5575420788515293No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAA2783040.49752686400864454No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTC2737290.48934809042709504No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTGGAATTCTC2703830.48336641252461093No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATT2277920.4072260528280483No Hit
TCGGGGGCCCGAGGTGGGATCCCGAGGCCTGGAATTCTC2269680.40575297972833313No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAAT2247190.40173241977534846No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGGAATTCT2086180.3729484998985117No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGA2057070.367744475877552No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAAT1967210.3516801131663381No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAA1759130.3144814419783858No Hit
TCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATT1651730.2952814358000598No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAAT1398460.2500041027946163No Hit
TGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC1395640.24949996855417983RNA PCR Primer, Index 1 (95% over 22bp)
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAA1345330.2405059991795841No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGG1340540.23964968605487105No Hit
GGGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTC1212060.21668118704377864No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATT1208820.2161019689802984No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAA1199770.21448409136471322No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGA1148600.2053363789238851No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAAT1100750.1967821862271169No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATT1098200.1963263201586371No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCTGGAAT1091240.1950820739481981No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGG1029430.18403223799026205No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAAT949980.16982888146448924No Hit
GGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTCT818960.14640630409498948No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAA804160.14376049318773412No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAAT775540.13864406696032547No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATT714710.12776942658949145No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCT674920.120656128211134No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTC657010.11745433947134053No Hit
CGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGGGTG645940.1154753444211164No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGCCTTGGA630390.11269545525842582No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCT601650.10755757651014752No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGG578370.10339578746143775No Hit
CGGGGGCCCGAGGTGGGATCCCGAGGCCTCTGGAATTCT561410.1003638311785289No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)