FastQCFastQC Report
Wed 27 Sept 2023
SRR10963471_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10963471_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49132721
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length18-36
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGGGATAACAGGGTAAT17435333.5486188521901725No Hit
TTTTCCACGTTCTAGCATTC8661231.7628231906797915No Hit
ACAATCGACCGATCCTGA3373520.6866137130894908No Hit
TTTCCACGTTCTAGCATTC3217510.6548609428734875No Hit
TCCGATATAGTGTAACGGCTATCACATCACG2979670.6064532839530707No Hit
AGAGATGTATTTATTAGATA2440780.49677281256212125No Hit
GTGCCCGAGTTGTAATTTG2315930.47136204811453447No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT2179860.44366767311747296No Hit
CCTGATTCAAGCTCAGGTA2116760.4308249079060775No Hit
AAGAGGTGCACAATCGACCGATCCTGA1997630.40657833707194846No Hit
ATGTATTTATTAGATAAAAAATCAATG1846000.3757170297977187No Hit
TTGCTGGCGAATTGCAATGTC1664690.33881494167603704No Hit
TTAGGGATAACAGGGTAAT1615670.32883788382084517No Hit
CCAACGGGGCCTTTCCTTCTGGC1580110.321600344503615No Hit
TCAGATTTAGGCTCTGATA1576690.3209042706997644No Hit
ATGTTAGGGATAACAGGGT1575640.32069056383016115No Hit
TTCCACGTTCTAGCATTC1568310.31919868634997844No Hit
AGAGATGTATTTATTAGAT1484190.3020777131394779No Hit
TTGCTCGAATATATTAGC1406910.2863488875366785No Hit
ATGTTAGGGATAACAGGGTAATGCGA1280110.26054123890268566No Hit
ACAGGGTAATCAACGCGA1265160.25749846014023936No Hit
ATGTATTTATTAGATAAAAAATCA1226580.2496462591599598No Hit
ACGTTCTAGCATTCAAGGTCC1170430.23821802989498586No Hit
TCTTCTTCATGATAAGACAGTT953790.19412521443703473No Hit
GTCTAGTGGTATGATTCTCGC873760.17783668036622682No Hit
TCAGGTCGAGGTCCTGATC849220.17284204552807078No Hit
TTGCCCGAGTTGTAATTTG837590.17047498753427476No Hit
TTGTGGCGTCGCTGAACCATAG821340.1671676193142244No Hit
ACGTTCTAGCATTCAAGGTCCCA816840.1662517327302105No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC807120.16427341770874038No Hit
CAAGAGGTGCACAATCGACCGATCCTGA799460.16271437521239662No Hit
AGGCGGCCAGGCCGGGAACCATCCCTGTGAGGC789470.1606811069958857No Hit
ATATAGATGGATACGAAT784730.159716373127391No Hit
ATGTATTTATTAGATAAAAAATC763570.15540967087900548No Hit
ACGTTCTAGCATTCAAGGTCCC762580.1552081758305224No Hit
TTTGTGGCGTCGCTGAACCATAG760030.1546891734329145No Hit
TCGGTGCCCGAGTTGTAATTTG753810.1534232146434552No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA747460.15213079690823555No Hit
TCACGTTCTAGCATTCAAGGTCCCA730140.1486056512115419No Hit
TTTTCCACGTTCTAGCATT728110.14819248459697562No Hit
ATGTACACTAGGGATAAC720870.14671892484847318No Hit
ATATAGATGGATACGAATAAGGCGTCC715530.14563207276877663No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC706910.14387764113450993No Hit
TTGTGGCGTCGCTGAACCATAGC695710.14159810119207522No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCAT680760.13855532242962892No Hit
TTTGTGGCGTCGCTGAACCATAGC674030.1371855631606481No Hit
ATATAGATGGATACGAATAAGGCGTC668010.1359603104415894No Hit
ATCCTGTCTGTGACGCCA627770.12777024907698475No Hit
ATGCGAAAGCAGTTGAAGACAAGTT625530.12731434108849782No Hit
AATATAGATGGATACGAAT606790.12350018229195978No Hit
ATGTATTTATTAGATAAAA599430.12200219890121697No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCA587810.11963717621094097No Hit
TCCAACGGGGCCTTTCCTTCTGGC582140.11848315911508342No Hit
TAGGGATAACAGGGTAAT559030.11377957268029182No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC557520.11347224184876713No Hit
AACAGGGTAATCAACGCGA548430.11162215094905899No Hit
TGCCCGAGTTGTAATTTG530320.10793621627428288No Hit
TGCTGGCGAATTGCAATGTC528070.10747827298227591No Hit
ATGTATTTATTAGATAAAAAATCAAT524810.1068147640347458No Hit
TCACGTTCTAGCATTCAAGGTCCC523450.1065379627560216No Hit
TCACGTTCTAGCATTCAAGGTCC523050.10645655061522034No Hit
AAGAGGTGCACAATCGACCGATCCTG502630.10230046082731709No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)