FastQCFastQC Report
Tue 26 Sept 2023
SRR10963472_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10963472_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43766392
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length18-37
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGGGATAACAGGGTAAT20692054.727840028485784No Hit
TTGCTGGCGAATTGCAATGTC3052050.6973501494023086No Hit
GTGCCCGAGTTGTAATTTG2722830.6221280474753321No Hit
TTAGGGATAACAGGGTAAT2024820.4626426596919389No Hit
AAGAGGTGCACAATCGACCGATCCTGA2000480.45708131481343034No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT1968470.44976748368931124No Hit
CCTGATTCAAGCTCAGGTA1764370.4031335276620472No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1663570.38010215692442734No Hit
ACAGGGTAATCAACGCGA1652100.37748142455973976No Hit
TTGTGGCGTCGCTGAACCATAG1521190.34757034575753926No Hit
ATGTTAGGGATAACAGGGT1502330.3432611031770679No Hit
TCAGATTTAGGCTCTGATA1435570.3280073897798109No Hit
TTTGTGGCGTCGCTGAACCATAG1432140.3272236834144336No Hit
ACAATCGACCGATCCTGA1260000.28789213422024823No Hit
TTTTCCACGTTCTAGCATTC1226400.28021501064104165No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC1210470.2765752315155428No Hit
TCACGTTCTAGCATTCAAGGTCCCA1164110.2659826288628041No Hit
TCACGTTCTAGCATTCAAGGTCCC1139100.260268198484353No Hit
TCTTCTTCATGATAAGACAGTT1065920.24354760611749762No Hit
TTGCCCGAGTTGTAATTTG1045400.23885907707448217No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA1040250.2376823750973121No Hit
TTGTGGCGTCGCTGAACCATAGC971930.22207222381959196No Hit
TTTGTGGCGTCGCTGAACCATAGC965770.22066475116340412No Hit
CAAGAGGTGCACAATCGACCGATCCTGA950710.21722375470200972No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC910880.20812316445915852No Hit
ATGTTAGGGATAACAGGGTAATGCGA886210.2024864192597827No Hit
GTCTAGTGGTATGATTCTCGC885090.20223051514047582No Hit
CACGTTCTAGCATTCAAGGTCCC876190.2001969913352693No Hit
TGCTGGCGAATTGCAATGTC866540.1979921031644555No Hit
TTCTAGCATTCAAGGTCCC840230.19198064121895175No Hit
ATGTACACTAGGGATAAC826960.18894863437680676No Hit
AGAGATGTATTTATTAGATA818890.18710475380287228No Hit
TTGCTGGCGAATTGCAATGT814430.18608570704206095No Hit
CACGTTCTAGCATTCAAGGTCCCA797100.18212604776742847No Hit
TCTAGCATTCAAGGTCCC795510.18176275531234104No Hit
TCACGTTCTAGCATTCAAGGTCC786620.17973151636534263No Hit
AGAGATGTATTTATTAGAT778590.17789677522424055No Hit
TCAGGTCGAGGTCCTGATC767800.17543141321770367No Hit
ACGTTCTAGCATTCAAGGTCCC751530.1717139489131295No Hit
AGGCGGCCAGGCCGGGAACCATCCCTGTGAGGC738790.16880303955601367No Hit
TCTAGCATTCAAGGTCCCA728980.1665615936538703No Hit
TTCTAGCATTCAAGGTCCCA720870.16470857364710348No Hit
ATCCTGTCTGTGACGCCA714160.16317543378947025No Hit
ACGTTCTAGCATTCAAGGTCC704230.1609065695888297No Hit
TAGGGATAACAGGGTAAT700880.16014114208911714No Hit
ACGTTCTAGCATTCAAGGTCCCA691580.15801622395558676No Hit
TCGGTGCCCGAGTTGTAATTTG680110.15539549159089924No Hit
ATGTTAGGGATAACAGGG663950.15170316072661416No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAT651640.1488905002724465No Hit
TGCCCGAGTTGTAATTTG647670.1479834115638319No Hit
AAGAGGTGCACAATCGACCGATCCTG644550.14727053580290558No Hit
CCACGTTCTAGCATTCAAGGTCCCA641240.1465142477360254No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCAT629940.14393235796087556No Hit
CACGTTCTAGCATTCAAGGTCC623220.14239693324503422No Hit
AAAGATTGGAAATCTTTTG622310.14218901114809737No Hit
CCACGTTCTAGCATTCAAGGTCCC620120.14168862720052408No Hit
TCCACGTTCTAGCATTCAAGGTCCCA611320.13967795197739855No Hit
CCAACGGGGCCTTTCCTTCTGGC609240.13920270147011432No Hit
TCCACGTTCTAGCATTCAAGGTCCC597320.13647915048606246No Hit
TGCTTGCTGGCGAATTGCAATGTC580880.13272284359195063No Hit
ATGTACACTAGGGATAACA566060.12933668372755058No Hit
TTTTCCACGTTCTAGCATT547050.12499316827395779No Hit
ATGTATTTATTAGATAAAAAATCA545250.12458189379650028No Hit
TTTCCACGTTCTAGCATTCAAGGTCCC540110.12340747667753833No Hit
ATGCGAAAGCAGTTGAAGACAAGTT520930.11902511863440789No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAT520460.1189177302986273No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA512580.11711726203064671No Hit
CCTGATTCAAGCTCAGGT507130.11587201430723375No Hit
ATATAGATGGATACGAATAAGGCGTC482220.11018043251086358No Hit
TGTGCCCGAGTTGTAATTTG480640.10981942491398422No Hit
TTTAGGGATAACAGGGTAA479390.10953381763797207No Hit
AACAGGGTAATCAACGCGA469680.10731522031790967No Hit
TTTCCACGTTCTAGCATTC468070.10694735814640603No Hit
TTGGGTAAAACCATACTGCAGACATCT440080.10055204002194194No Hit

[OK]Adapter Content

Adapter graph