FastQCFastQC Report
Wed 30 Aug 2023
SRR10963473_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10963473_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126754446
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length18-37
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCCCGAGTTGTAATTTG36941512.9144153255184437No Hit
TCACGTTCTAGCATTCAAGGTC23548211.8577817775322847No Hit
TTGCCCGAGTTGTAATTTG18987191.497950612320139No Hit
CACGTTCTAGCATTCAAGGTC18077811.4262071722517726No Hit
ACGTTCTAGCATTCAAGGTC16590121.3088392970452492No Hit
TGCCCGAGTTGTAATTTG12421630.9799758818716306No Hit
TCAGATTTAGGCTCTGATA11281250.8900082289815696No Hit
TCACGTTCTAGCATTCAAGGTCC9095820.7175937639299848No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAT8274680.6528118153741133No Hit
AAGAGGTGCACAATCGACCGATCCTGA8079690.6374285285425018No Hit
ACGTTCTAGCATTCAAGGTCC7513650.5927721067866921No Hit
AAGAGGTGCACAATCGACCGATCCTG7227050.5701614600563991No Hit
TCACGTTCTAGCATTCAAGGTCCC7065620.5574258121091863No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT6849060.5403408098205881No Hit
CACGTTCTAGCATTCAAGGTCC6693820.5280935076628397No Hit
ATCCTGTCTGTGACGCCA6521110.5144679501025156No Hit
TGTGCCCGAGTTGTAATTTG6161230.4860760465948469No Hit
AGATGGATACGAATAAGGCGTC6134500.4839672448254793No Hit
TTTAGGGATAACAGGGTAAT5931760.46797253959833485No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAT5867460.4628997392328155No Hit
ATGGCTGGAAGAGGCCAGCA5469220.43148151189899875No Hit
TTGTGGCGTCGCTGAACCATA5085140.401180405143343No Hit
ATGGAGAACAGAAATCTCCAGTAGAAC4950670.39057170428562327No Hit
CACGTTCTAGCATTCAAGGTCCC4944470.3900825695691968No Hit
ACGTTCTAGCATTCAAGGTCCC4826480.38077402034481694No Hit
TTGTGGCGTCGCTGAACCATAG4646290.36655834541693316No Hit
TAGATGGATACGAATAAGGCGTC4370150.34477291628886925No Hit
TCGGTGCCCGAGTTGTAATTTG4357810.34379938041778824No Hit
AGACACCACAAAAGGTGTTAGTTC3822660.301579954047529No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA3765570.2970759700215959No Hit
TCTTCTTCATGATAAGACAGTT3471990.2739146522718422No Hit
ATATAGATGGATACGAATAAGGCGTC3463730.2732629986012483No Hit
AAAGATTGGAAATCTTTTG3286020.2592429775599351No Hit
ATTGCGCCCCTTGGTATTCCAGGGGGCATGC2985700.23554992303780808No Hit
TGTGGCGTCGCTGAACCATAG2942170.2321157239723173No Hit
TCCGATATAGTGTAACGGCTATCACATCACG2933240.23141121219527083No Hit
ATCCCGTAGATTATTCACACGTT2867720.2262421627403902No Hit
TTGCTCGAATATATTAGC2844260.2243913400875895No Hit
ATGCCTTGCTAAGGCATTG2769240.21847281001882965No Hit
TCCATGACTATGCCATTTCTAGGTAGCCTATGGTA2718230.21444849358577922No Hit
TGTGGCGTCGCTGAACCATA2643500.20855284239891672No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAT2525700.19925928278681443No Hit
ATTGCGCCCCTTGGTATTCCAGGGGGC2520900.19888059784506493No Hit
ACAATCGACCGATCCTGA2512770.1982392002249767No Hit
GTGCCCGAGTTGTAATTT2488450.19632052985344592No Hit
ATGGATACGAATAAGGCGTC2098120.16552634374655387No Hit
GTCTAGTGGTATGATTCTCGC2086210.16458673173483793No Hit
CCACGTTCTAGCATTCAAGGTC2010660.1586263885370932No Hit
ATGGAGAACAGAAATCTCCAGT1938940.1529682043657861No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGC1905600.15033792187455103No Hit
ATAGATGGATACGAATAAGGCGTC1896200.14959633053029162No Hit
ATGATGCCCTGACGAGTAGGC1895950.14957660735624217No Hit
TCAGGTCGAGGTCCTGATC1836960.14492272720753321No Hit
TTGCTCGAATATATTAGCATG1799910.14199975281340427No Hit
TTTCGGTGCCCGAGTTGTAATTTG1720010.13569622638719905No Hit
TTGCTCGAATATATTAGCA1704290.13445603320296945No Hit
TCCCGCTAGAGACCACCA1673540.13203008279488673No Hit
AGAGCATTGTAATTATTGCTCTTC1636220.12908580737278438No Hit
ATCCCTTAGCTCTCACCA1621720.12794186327791612No Hit
ATTAGCATGGAATAATAGAAT1583960.12496287506948672No Hit
ATCCCGTAGATTATTCACACGTTT1573320.12412345678194199No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAT1517320.11970546579486452No Hit
AGAGGTGCACAATCGACCGATCCTGA1462540.11538372389714835No Hit
CCTGATTCAAGCTCAGGTA1457120.1149561254837562No Hit
TTTGTGGCGTCGCTGAACCATA1444040.11392420901748881No Hit
TATAGATGGATACGAATAAGGCGTC1369430.10803802495417006No Hit
CCATTCATGCCTTTCTGAAG1363980.10760805975989196No Hit
ATAAATCATTTGTATACGACTTAGAT1355530.10694141647702045No Hit
TCAGATTTAGGCTCTGAT1344220.10604914008302321No Hit
AGAGGTGCACAATCGACCGATCCTG1288080.10162010411847802No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)