FastQCFastQC Report
Thu 24 Aug 2023
SRR10963474_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10963474_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126167989
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length18-37
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCCCGAGTTGTAATTTG36180172.8676188220769694No Hit
CACGTTCTAGCATTCAAGGTC35890302.8446438977481048No Hit
TCACGTTCTAGCATTCAAGGTC35849332.8413966398402373No Hit
GTGCCCGAGTTGTAATTTG30123612.387579467562093No Hit
ACGTTCTAGCATTCAAGGTC27947432.2150967310733627No Hit
TGCCCGAGTTGTAATTTG22963821.8200987573797345No Hit
TTTAGGGATAACAGGGTAAT17019201.348931700892847No Hit
TCACGTTCTAGCATTCAAGGTCC12741201.0098599574254925No Hit
CACGTTCTAGCATTCAAGGTCC12155630.9634480264245157No Hit
ACGTTCTAGCATTCAAGGTCC12034410.9538402010988699No Hit
TCACGTTCTAGCATTCAAGGTCCC11829420.9375928152425415No Hit
AAGAGGTGCACAATCGACCGATCCTGA11002990.8720904634534516No Hit
CACGTTCTAGCATTCAAGGTCCC10286470.8152995130959884No Hit
AAGAGGTGCACAATCGACCGATCCTG10018450.7940564068117152No Hit
GTGCCCGAGTTGTAATTT9554660.7572966864043462No Hit
ACGTTCTAGCATTCAAGGTCCC9088790.720372106430261No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAT9035640.7161594689442186No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAT6982160.5534018617036053No Hit
TCAGATTTAGGCTCTGATA6206440.4919187544472949No Hit
ATATAGATGGATACGAATAAGGCGTC5835680.4625325366801242No Hit
TAGATGGATACGAATAAGGCGTC5654720.4481897543758108No Hit
TGTGCCCGAGTTGTAATTTG5235670.41497609984098266No Hit
AGATGGATACGAATAAGGCGTC4815970.38171092669155565No Hit
ATCCTGTCTGTGACGCCA4748680.37637756118947097No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT4422410.35051759444307223No Hit
TTGTGGCGTCGCTGAACCATA3983900.3157615518465623No Hit
TTGTGGCGTCGCTGAACCATAG3884740.3079021890409936No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA3667710.2907005199234807No Hit
GTCTAGTGGTATGATTCTCGC3631610.287839255328069No Hit
TTCTAGCATTCAAGGTCCC3407550.2700803925788181No Hit
CCACGTTCTAGCATTCAAGGTC3139640.24884600482932323No Hit
AAAGATTGGAAATCTTTTG3073820.24362915065563898No Hit
TCGGTGCCCGAGTTGTAATTTG3063980.24284923809001982No Hit
TCTTCTTCATGATAAGACAGTT3001910.23792960669286722No Hit
TCTAGCATTCAAGGTCCC2957180.2343843334143972No Hit
ATGCCTTGCTAAGGCATTG2753900.21827248114416725No Hit
ATAGATGGATACGAATAAGGCGTC2541100.20140607931858215No Hit
TGTGGCGTCGCTGAACCATAG2496120.19784099118834333No Hit
ATTAGCATGGAATAATAGAAT2440540.1934357533431083No Hit
TCCATGACTATGCCATTTCTAGGTAGCCTATGGTA2440150.193404842174349No Hit
ACAATCGACCGATCCTGA2393440.18970263526987022No Hit
TTCTAGCATTCAAGGTCC2285720.18116481193973855No Hit
TATAGATGGATACGAATAAGGCGTC2108360.1671073635008956No Hit
TAGCATGGAATAATAGAAT2053130.16272986644813686No Hit
TGTGGCGTCGCTGAACCATA2029510.16085775925302256No Hit
TCCGATATAGTGTAACGGCTATCACATCACG2011710.15944694180708546No Hit
AGAGGTGCACAATCGACCGATCCTGA1971890.1562908322173543No Hit
GTCTAGTGGTATGATTCTCG1931180.1530641817553262No Hit
TTTAGCATGGAATAATAGAAT1903180.15084491835722294No Hit
ATCCCGTAGATTATTCACACGTT1845200.1462494579350076No Hit
TTGCTCGAATATATTAGC1776180.14077897365868294No Hit
AGAGGTGCACAATCGACCGATCCTG1773130.14053723246710384No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAT1726200.13681758849306855No Hit
CCACGTTCTAGCATTCAAGGTCCC1641800.1301280945359286No Hit
ACAGGGTAATCAACGCGA1597440.12661214723807637No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGC1499420.11884314015657331No Hit
TGTGCCCGAGTTGTAATTT1482300.11748621910744729No Hit
CCACGTTCTAGCATTCAAGGTCC1458610.11560856375383774No Hit
ATGGATACGAATAAGGCGTC1456210.11541834117685748No Hit
ATGTTAGGGATAACAGGGTAATGCGA1405210.11137611141602646No Hit
CTGCCCGAGTTGTAATTTG1385160.10978696030417034No Hit
GTTCTAGCATTCAAGGTC1320050.10462638030950941No Hit
ATGGCTGGAAGAGGCCAGCA1293890.10255295422042432No Hit
TTAGGGATAACAGGGTAAT1273680.1009511216034362No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)