FastQCFastQC Report
Thu 24 Aug 2023
SRR10970363_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10970363_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11121557
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAAC269209324.206080137879976Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA8747547.865391509480192Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG7592926.827209535499391TruSeq Adapter, Index 11 (100% over 49bp)
AGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACT4779884.297851460906058Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTGA2200601.9786797837748795Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAAC1822551.63875435786554Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAA1641231.4757196317026475Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG1229531.1055376508882704Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGTGACTCTAGATAACCTAGATCGGAAGAGCACACGTCTGAACTCCAG1034370.9300586239858322Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGCAATTTTTGACAGTCTCAGATCGGAAGAGCACACGTCTGAACTCCAG891480.8015784120874442Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA696800.6265309794303082Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCTGAACT585450.5264101060669832Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGGTGACTCTAGATAACCTCAGATCGGAAGAGCACACGTCTGAACTCCA505980.4549542838291437Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACT389970.35064334966767696Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG386560.34757723221667614Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC379180.34094147069515535No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT372730.3351419230239075Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCTTGACTAGCTTGCTGTTTAGATCGGAAGAGCACACGTCTGAACTCCA341670.3072141787341467Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAA341590.30714224635992965Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTGAA328140.2950486159446919Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGATCATCGACACTTCGAACGAGATCGGAAGAGCACACGTCTGAACTC298950.2688022909022541Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGCAATTTTTGACAGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT295200.26543046086083094Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTC289400.26021536373009646Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA269680.24248403348559922Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT267600.24061379175595649Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA253640.22806159245508517Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC244170.21954659765714457TruSeq Adapter, Index 11 (100% over 50bp)
CAAGCATCAAGCACGCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT240260.2160309028672874Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTGGTGACTCTAGATAACCTCGAGATCGGAAGAGCACACGTCTGAACTCC226240.20342475428575335Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTGGTGACTCTAGATAACCTAGATCGGAAGAGCACACGTCTGAACTCCA215420.19369590067290038Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC207820.1868623251222828Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAAC203210.18271722205802657Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG199110.17903068787940396Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTGGTGACTCTAGATAACCTCAGATCGGAAGAGCACACGTCTGAACTCC190730.17149577168017033Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGT188450.16944569901498505TruSeq Adapter, Index 11 (100% over 46bp)
GGGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAAC186880.1680340261709759Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC181170.16289985296123555No Hit
TTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC178340.1603552452233082Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCTGAACTCCA171830.15450174827139762Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGATCATCGACACTTCGAACGAGATCGGAAGAGCACACGTCTGAACTCC166970.15013185653771322Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCTGAACTC163400.14692187433827836Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCGGTTTCTATCTACTTCAAATTCCAGATCGGAAGAGCACACGTCTGAA159780.14366693440495787Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCATGATCACGAAGGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAG158670.1426688727126966Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACAAGCATCAAGCACGCAGCAGATCGGAAGAGCACACGTCTGAACTCCAG151690.13639277306226097Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC144090.12955919751164338No Hit
GCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG138580.1246048552374456TruSeq Adapter, Index 11 (100% over 45bp)
GGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG135260.12161966170743897TruSeq Adapter, Index 11 (100% over 45bp)
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC134340.12079243940394317Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG130140.11701598975754923TruSeq Adapter, Index 11 (100% over 45bp)
GAGGGCGCACCACCGGCCCGTCTAGATCGGAAGAGCACACGTCTGAACTC129300.11626069982827043Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGAACTC125900.11320357392404679Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGGGCGCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTA121300.10906746240656771TruSeq Adapter, Index 11 (100% over 38bp)
GAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG119910.10781763740454686TruSeq Adapter, Index 11 (100% over 45bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC119820.10773671348355271TruSeq Adapter, Index 11 (100% over 50bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTAT119700.10762881492222716TruSeq Adapter, Index 11 (100% over 48bp)
GGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG115080.10347472031119384TruSeq Adapter, Index 11 (100% over 45bp)
GGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG114380.10284531203679484TruSeq Adapter, Index 11 (100% over 45bp)
TCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACTC113200.10178430951709369Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)