FastQCFastQC Report
Thu 24 Aug 2023
SRR10970365_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10970365_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11326473
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG163359314.42278633428076TruSeq Adapter, Index 10 (100% over 49bp)
GAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAAC152707413.48234353271314Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA4758254.200998845801336Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACT2874822.538142279595775Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTGA1191061.0515718352924162Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAAC1055820.9321701468762607Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAA1052820.9295214847552278Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG808470.7137879549971116Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGTGACTCTAGATAACCTAGATCGGAAGAGCACACGTCTGAACTCCAG674580.5955781645354207Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGCAATTTTTGACAGTCTCAGATCGGAAGAGCACACGTCTGAACTCCAG518880.45811260045382174Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC510430.45065220214624624No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC509200.4495662506766228TruSeq Adapter, Index 10 (100% over 50bp)
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA378630.3342876462955414Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT369950.3266241838920201Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGT315690.27871871499627465TruSeq Adapter, Index 10 (100% over 46bp)
GAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCTGAACT311230.2747810373096726Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACT283840.2505987521446438Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGGTGACTCTAGATAACCTCAGATCGGAAGAGCACACGTCTGAACTCCA283630.25041334579617147Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG277980.24542503213489317TruSeq Adapter, Index 10 (100% over 45bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC269980.23836193314547258TruSeq Adapter, Index 10 (100% over 50bp)
GGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG265140.2340887582568731TruSeq Adapter, Index 10 (100% over 45bp)
GGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG260180.22970963688343232TruSeq Adapter, Index 10 (100% over 45bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG253070.22343230765658473Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC244970.21628091992979634Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG236370.20868808851616916TruSeq Adapter, Index 10 (100% over 45bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTAT233780.2064014102183442TruSeq Adapter, Index 10 (100% over 48bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC233120.20581870455171702TruSeq Adapter, Index 10 (98% over 50bp)
GGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGT232750.2054920362234563TruSeq Adapter, Index 10 (100% over 46bp)
GGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG231060.20399995656194123TruSeq Adapter, Index 10 (100% over 45bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCG220670.19482675674943117TruSeq Adapter, Index 10 (100% over 50bp)
GGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG218750.19313161299197024TruSeq Adapter, Index 10 (100% over 45bp)
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT218100.1925577361990798Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG212290.18742816055801306TruSeq Adapter, Index 10 (100% over 45bp)
TGGCAATTTTTGACAGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT200280.17682468320014536Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATTGATCATCGACACTTCGAACGAGATCGGAAGAGCACACGTCTGAACTC192050.1695585201147789Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAAGCATCAAGCACGCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT180720.15955540617101194Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA179170.1581869307418117Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG177980.15713629476713536TruSeq Adapter, Index 10 (100% over 45bp)
CGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGT175380.15484078759557368TruSeq Adapter, Index 10 (100% over 46bp)
GGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTC174530.15409033332794772Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA173640.15330456356537467Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCTTGACTAGCTTGCTGTTTAGATCGGAAGAGCACACGTCTGAACTCCA168700.14894309993940744Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC167630.14799841044957243Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC165750.14633858218705859TruSeq Adapter, Index 10 (100% over 44bp)
CCTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAA161440.14253333760650821Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG161000.1421448671620901TruSeq Adapter, Index 10 (100% over 45bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTAT157800.13931962756632182TruSeq Adapter, Index 10 (100% over 48bp)
CGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTGAA157660.13919602333400696Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGTGACTCTAGATAACCTCGAGATCGGAAGAGCACACGTCTGAACTCC154860.13672393868770977Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG154340.13626483725339741Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG150260.1326626567687929TruSeq Adapter, Index 10 (100% over 45bp)
TTTGGTGACTCTAGATAACCTAGATCGGAAGAGCACACGTCTGAACTCCA149170.13170030953148434Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG148010.13067616017801834TruSeq Adapter, Index 10 (100% over 45bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC146890.12968732631949947No Hit
ACCATGATCACGAAGGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAG142990.1262440655621569Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG142320.12565253102179294TruSeq Adapter, Index 10 (100% over 45bp)
CGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGT141710.12511396972384958TruSeq Adapter, Index 10 (100% over 46bp)
GGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC140410.12396621613806875TruSeq Adapter, Index 10 (100% over 44bp)
GGGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATC138090.12191791743113678TruSeq Adapter, Index 10 (100% over 42bp)
GCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGT134630.11886312711821236TruSeq Adapter, Index 10 (100% over 46bp)
AGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGT133790.11812150172432317TruSeq Adapter, Index 10 (100% over 46bp)
GGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG133640.11798906861827155TruSeq Adapter, Index 10 (100% over 45bp)
TAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC133620.117971410870798No Hit
TTAGCAGTAAACTGAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGT130690.11538455086592268Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGT127530.11259462676510155TruSeq Adapter, Index 10 (100% over 46bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTC124320.10976055829559651TruSeq Adapter, Index 10 (100% over 50bp)
GGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG123960.10944271884107258TruSeq Adapter, Index 10 (100% over 45bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTAT123360.10891298641686606TruSeq Adapter, Index 10 (100% over 48bp)
AACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCTGAACTCCA122820.10843622723508016Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC115230.10173511206886733TruSeq Adapter, Index 10 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)