FastQCFastQC Report
Thu 24 Aug 2023
SRR10970367_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10970367_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8809303
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG204194423.17940477243205TruSeq Adapter, Index 9 (100% over 49bp)
GAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAAC115447613.105191182548722Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA2880583.2699295279093024Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACT2434692.7637714357197156Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC737160.8367971904247136TruSeq Adapter, Index 9 (100% over 50bp)
AGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAAC730070.828748880586807Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAA556370.6315709653760349Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTGA541770.6149975769933217Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGT383410.43523307122027705TruSeq Adapter, Index 9 (100% over 46bp)
GGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG362470.41146274569055014TruSeq Adapter, Index 9 (100% over 45bp)
AACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG359720.40834104582394315Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG330440.3751034559714883TruSeq Adapter, Index 9 (100% over 45bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT307940.3495622752447044TruSeq Adapter, Index 9 (100% over 48bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT272160.3089461220711786TruSeq Adapter, Index 9 (100% over 48bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG271690.3084125951848858Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG253450.2877072113423729TruSeq Adapter, Index 9 (100% over 45bp)
GGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGT250810.2847103794704303TruSeq Adapter, Index 9 (100% over 46bp)
GCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG246730.2800789120319735TruSeq Adapter, Index 9 (100% over 45bp)
GAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCTGAACT235130.26691101441283155Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT233930.26554881810740305Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC228620.25952109945588203TruSeq Adapter, Index 9 (100% over 50bp)
GGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG220090.24983815405146126TruSeq Adapter, Index 9 (100% over 45bp)
GGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTC218290.2477948595933186TruSeq Adapter, Index 9 (100% over 44bp)
CGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG214480.24346988632358316TruSeq Adapter, Index 9 (100% over 45bp)
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA203750.23128958102587688Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG203200.23066524105255548TruSeq Adapter, Index 9 (100% over 45bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC194790.22111851527867754TruSeq Adapter, Index 9 (98% over 50bp)
GGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG194710.221027702191649TruSeq Adapter, Index 9 (100% over 45bp)
CGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGT188760.21427347884389944TruSeq Adapter, Index 9 (100% over 46bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC181920.20650895990295712No Hit
GGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTC178640.20278562333478595TruSeq Adapter, Index 9 (100% over 44bp)
GGGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATC173890.1973935962924649TruSeq Adapter, Index 9 (100% over 42bp)
GAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG173080.1964741137863007TruSeq Adapter, Index 9 (100% over 45bp)
CTCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACT166380.18886851774765837Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTC165050.18735875017580847Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG163080.18512247790773004TruSeq Adapter, Index 9 (100% over 45bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCG159920.1815353609701017TruSeq Adapter, Index 9 (100% over 50bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT158900.1803774941104875TruSeq Adapter, Index 9 (100% over 48bp)
GGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG155630.17666550917819493TruSeq Adapter, Index 9 (100% over 45bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT155280.17626820192244494TruSeq Adapter, Index 9 (100% over 48bp)
AGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGT152580.1732032602352309TruSeq Adapter, Index 9 (100% over 46bp)
CGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGT145060.16466683005454574TruSeq Adapter, Index 9 (100% over 46bp)
GACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG142350.1615905367314531TruSeq Adapter, Index 9 (100% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCCGTCACGATCAGATCTCGTATG139630.1585028917724819TruSeq Adapter, Index 9 (97% over 49bp)
GGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG138800.1575607059945605TruSeq Adapter, Index 9 (100% over 45bp)
GGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGT133340.15136271280486094TruSeq Adapter, Index 9 (100% over 46bp)
GGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTC131500.14927401180320393TruSeq Adapter, Index 9 (100% over 44bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTC130960.14866102346576116TruSeq Adapter, Index 9 (100% over 50bp)
GCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGT129500.14700368462748983TruSeq Adapter, Index 9 (100% over 46bp)
GCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG126000.1430306120699901TruSeq Adapter, Index 9 (100% over 45bp)
GCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTA124360.1411689437859045TruSeq Adapter, Index 9 (100% over 47bp)
GACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTC121210.13759317848415475TruSeq Adapter, Index 9 (100% over 44bp)
TTGGTGACTCTAGATAACCTAGATCGGAAGAGCACACGTCTGAACTCCAG119070.13516392840614067Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG118430.13443742370991213TruSeq Adapter, Index 9 (97% over 49bp)
GGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGT117980.13392660009537644TruSeq Adapter, Index 9 (100% over 46bp)
GAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG108950.12367607289702715TruSeq Adapter, Index 9 (100% over 45bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGT108630.12331282054891289TruSeq Adapter, Index 9 (100% over 50bp)
ACATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG106420.12080410901974879TruSeq Adapter, Index 9 (97% over 49bp)
GACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGT103410.11738726662029902TruSeq Adapter, Index 9 (100% over 46bp)
GAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGT102240.11605912522250625TruSeq Adapter, Index 9 (100% over 46bp)
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT101430.11513964271634204Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGT100640.11424286348193495TruSeq Adapter, Index 9 (100% over 46bp)
GGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCT99870.113368787519285TruSeq Adapter, Index 9 (100% over 43bp)
GGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTA99140.11254011810014936TruSeq Adapter, Index 9 (100% over 47bp)
GACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG98750.11209740430088509TruSeq Adapter, Index 9 (100% over 45bp)
CGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG98300.11158658068634941TruSeq Adapter, Index 9 (100% over 45bp)
GTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGT96760.10983842876104953TruSeq Adapter, Index 9 (100% over 46bp)
GGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG94770.10757945322121397TruSeq Adapter, Index 9 (100% over 45bp)
GGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG93340.10595616929057838TruSeq Adapter, Index 9 (100% over 45bp)
ATATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG92040.1044804566263642TruSeq Adapter, Index 9 (97% over 49bp)
TGGCAATTTTTGACAGTCTCAGATCGGAAGAGCACACGTCTGAACTCCAG89360.10143821821090727Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAAC88370.10031440625892878Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)